Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 微生物代谢工程团队

	Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli
	Bao, Xue 1,2; Liu, Aiqiu 1,2; Lu, Xuefeng 1; Li, Jian-Jun 1
刊名	BIOTECHNOLOGY LETTERS
	2012-08-01
卷号	34 期号:8 页码:1537-1543
关键词	Escherichia coli Hydrogen peroxide stability Overexpression Pleurotus eryngii Peroxidase
英文摘要	The vpl2 gene, encoding versatile peroxidase (VP) from Pleurotus eryngii, was synthesized with codon optimization and cloned into vector-pET-32a(+) and over-expressed in Escherichia coli BL21(DE3). An active peroxidase fused to the thioredoxin-hexahistidine tag was directly obtained by IPTG induction in the presence of hemin. Most of over-expressed protein was in the soluble form, and was purified on a nickel column with > 85 % purity at a yield of 12.5 mg/l. The purified fusion protein, having an Rz value (A(407)/A(280), a measure of hemin content of the peroxidases) of 1.2, oxidized ABTS veratryl alcohol, Mn2+, and Reactive Black 5. Activity of the enzyme increased after removing the tag. It lost only 5 % of its activity in 6.4 mM H2O2. This is the first report on direct over-expression of active VP in E. coli.
学科主题	生物代谢工程
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	PHANEROCHAETE-CHRYSOSPORIUM ; MANGANESE PEROXIDASE ; RECOMBINANT ; INACTIVATION ; MUTAGENESIS ; ENZYME ; PH
收录类别	SCI
语种	英语
WOS记录号	WOS:000306789200019
公开日期	2014-03-26
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1736]
专题	青岛生物能源与过程研究所_微生物代谢工程团队
作者单位	1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Bao, Xue,Liu, Aiqiu,Lu, Xuefeng,et al. Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2012,34(8):1537-1543.
APA	Bao, Xue,Liu, Aiqiu,Lu, Xuefeng,&Li, Jian-Jun.(2012).Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli.BIOTECHNOLOGY LETTERS,34(8),1537-1543.
MLA	Bao, Xue,et al."Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli".BIOTECHNOLOGY LETTERS 34.8(2012):1537-1543.