Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite

	Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite
	Zhang Z ; Luo LQ ; Zhu LM ; Ding YP ; Deng DM ; Wang ZX
刊名	analyst
	2013
卷号	138 期号:18 页码:5365-5370
ISSN号	0003-2654
通讯作者	luo lq
中文摘要	a novel protocol of electrochemical thrombin aptasensor based on a gold nanoparticles/thionine-graphene (aunps/th-g) nanocomposite modified glassy carbon electrode was presented. graphene was non-covalently functionalized by thionine via pi-stacking interaction, and then aunps were electrodeposited onto the th-g surface. the morphology and conductivity of the aunps/th-g nanocomposite were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. the ethanethiol-substituted oligonucleotide probe was immobilized onto the surface of the aunps/th-g nanocomposite to prepare an electrochemical biosensing platform. the aptamer-thrombin reaction was monitored by differential pulse voltammetry. under optimum conditions, the proposed biosensor exhibits high sensitivity and a low detection limit for thrombin determination. the thrombin could be quantified in a wide range of 0.5 to 40 nm and with a low detection limit of 0.093 nm (s/n = 3).
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000323115200026
公开日期	2014-04-16
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/50207]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhang Z,Luo LQ,Zhu LM,et al. Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite[J]. analyst,2013,138(18):5365-5370.
APA	Zhang Z,Luo LQ,Zhu LM,Ding YP,Deng DM,&Wang ZX.(2013).Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite.analyst,138(18),5365-5370.
MLA	Zhang Z,et al."Aptamer-linked biosensor for thrombin based on AuNPs/thionine-graphene nanocomposite".analyst 138.18(2013):5365-5370.