Single molecular dissection of the ligand binding property of epidermal growth factor receptor

	Single molecular dissection of the ligand binding property of epidermal growth factor receptor
	Zhang J ; Liu HQ ; Zhu R ; Hinterdorfer P ; Zhang BL ; Tang JL
刊名	analyst
	2013
卷号	138 期号:18 页码:5325-5331
ISSN号	0003-2654
通讯作者	zhang bl
中文摘要	the understanding of ligand binding interactions is an important component of understanding the fundamental mechanism of receptor function. in this study, the binding abilities of egf and tgf-alpha to egfr on human bladder cancer (t24) cells were investigated by single molecular force spectroscopy (smfs) based on atomic force microscopy (afm). by approaching the specifically functionalized afm tips to the t24 cell surface and subsequent retraction, specific unbinding events of the egf/egfr complexes and tgf-alpha/egfr complexes were investigated. further, the unbinding forces and the kinetic off rate constants that govern the bond stabilities were calculated through varying the external mechanic forces applied. meanwhile, the distances from the energy minimum to the transition states along the separation paths of the egf/egfr complexes and tgf-alpha/egfr complexes were deduced. this study at single-molecule level may enrich our understanding of the ligand binding properties of egfr and provide some new information to the development of improved egfr inhibitors. in addition, the results present new insight into the study of the energy landscape of the dissociation of ligand-egfr system.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000323115200021
公开日期	2014-04-16
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/50206]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhang J,Liu HQ,Zhu R,et al. Single molecular dissection of the ligand binding property of epidermal growth factor receptor[J]. analyst,2013,138(18):5325-5331.
APA	Zhang J,Liu HQ,Zhu R,Hinterdorfer P,Zhang BL,&Tang JL.(2013).Single molecular dissection of the ligand binding property of epidermal growth factor receptor.analyst,138(18),5325-5331.
MLA	Zhang J,et al."Single molecular dissection of the ligand binding property of epidermal growth factor receptor".analyst 138.18(2013):5325-5331.