Label-free G-quadruplex-specific fluorescent probe for sensitive detection of copper(II) ion

	Label-free G-quadruplex-specific fluorescent probe for sensitive detection of copper(II) ion
	Zhang LB ; Zhu JB ; Ai J ; Zhou ZX ; Jia XF ; Wang EK
刊名	biosensors & bioelectronics
	2013
卷号	39 期号:1 页码:268-273
关键词	ATOMIC-ABSORPTION SPECTROMETER PROTOPORPHYRIN-IX AQUEOUS-SOLUTION EMISSION-SPECTROMETRY STRUCTURAL-CHANGES CATALYTIC BEACON POTASSIUM-ION DNA BINDING DNAZYME
ISSN号	0956-5663
通讯作者	wang ek
中文摘要	an effective g-quadruplex-based probe has been constructed for rapid and sensitive detection of cu2+. in this probe, an anionic porphyrin, protoporphyrin ix (ppix) served as a reference signal, which binds to g-quadruplex specifically and the fluorescence intensity increases sharply. while, in the presence of cu2+, the g-quadruplex can catalyze the related cu2+ insertion into the protoporphyrin, the fluorescent intensity is decreased. the fluorescence of the response ligand could be selectively quenched in the presence of cu2+ and not interfered by other metal ions. the probe provided an effective platform for reliable detection of cu2+ with a detection limit as low as 3.0 nm, the high sensitivity was attributed to the strong metalation of ppix with cu2+ catalyzed by g-quadruplex (ps5.m). linear correlations were obtained over the logarithm of copper ion concentration in the range from 8 x 10(-9) m to 2 x 10(-6) m (r=0.998). the g-quadruplex-based probe also could be used to detect cu2+ in real water samples. additionally, these striking properties endow the g-quadruplex-ligand with a great promise for analytical applications. (c) 2012 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000309486300044
公开日期	2014-04-16
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/50106]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhang LB,Zhu JB,Ai J,et al. Label-free G-quadruplex-specific fluorescent probe for sensitive detection of copper(II) ion[J]. biosensors & bioelectronics,2013,39(1):268-273.
APA	Zhang LB,Zhu JB,Ai J,Zhou ZX,Jia XF,&Wang EK.(2013).Label-free G-quadruplex-specific fluorescent probe for sensitive detection of copper(II) ion.biosensors & bioelectronics,39(1),268-273.
MLA	Zhang LB,et al."Label-free G-quadruplex-specific fluorescent probe for sensitive detection of copper(II) ion".biosensors & bioelectronics 39.1(2013):268-273.