Versatile Biofunctionalization of Polypeptide-Based Thermosensitive Hydrogels via Click Chemistry

	Versatile Biofunctionalization of Polypeptide-Based Thermosensitive Hydrogels via Click Chemistry
	Cheng YL ; He CL ; Xiao CS ; Ding JX ; Cui HT ; Zhuang XL ; Chen XS
刊名	biomacromolecules
	2013
卷号	14 期号:2 页码:468-475
关键词	BLOCK-COPOLYMER HYDROGELS DRUG-DELIVERY INJECTABLE HYDROGEL AQUEOUS-SOLUTIONS SECONDARY STRUCTURE TEMPERATURE GELATION ACID) SOL NANOPARTICLES
ISSN号	1525-7797
通讯作者	chen xs
中文摘要	in this study, we report thermosensitive hydrogels based on poly(ethylene glycol)-block-poly(gamma-propargyl-l-glutamate) (peg-pplg). c-13 nmr spectra, dls, and circular dichroism spectra were employed to study the mechanism of the sol-gel phase transition. mouse fibroblast l929 cells were encapsulated and cultured within the hydrogel matrices, and the encapsulated cells were shown to be highly viable in the gel matrices, suggesting that the hydrogels have excellent cytocompatibilities. the mass loss of the hydrogels in vitro was accelerated by the presence of proteinase k compared to the control group. in vivo biocompatibility studies revealed that the in situ formed gels in the subcutaneous layer last for similar to 21 days, and h&e staining study suggested acceptable biocompatibility of our materials in vivo. the presence of alkynyl side groups in the peg-pplg copolymers allowed convenient further functionalization with azide-modified bioactive molecules, such as biotin and galactose. the biofunctionalized pegpolypeptide block copolymers showed sol-gel phase transitions similar to the parent copolymers. interestingly, the incorporation of galactose groups into the hydrogels was found to improve cell adhesion, likely due to the adsorption of fibronectin (fn) in cell-extracellular matrix (ecm). because bioactive materials have shown unique advantages in biomedical applications, especially tissue engineering and regenerative medicine applications, we believe our novel functionalizable thermosensitive hydrogels have potential to serve as a versatile platform for the development of new biofunctional materials, for example, bioadhesive and bioresponsive hydrogels.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000314908500021
公开日期	2014-04-16
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/50062]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Cheng YL,He CL,Xiao CS,et al. Versatile Biofunctionalization of Polypeptide-Based Thermosensitive Hydrogels via Click Chemistry[J]. biomacromolecules,2013,14(2):468-475.
APA	Cheng YL.,He CL.,Xiao CS.,Ding JX.,Cui HT.,...&Chen XS.(2013).Versatile Biofunctionalization of Polypeptide-Based Thermosensitive Hydrogels via Click Chemistry.biomacromolecules,14(2),468-475.
MLA	Cheng YL,et al."Versatile Biofunctionalization of Polypeptide-Based Thermosensitive Hydrogels via Click Chemistry".biomacromolecules 14.2(2013):468-475.