Studies on the interactions between ginsenosides and liposome by equilibrium dialysis combined with ultrahigh performance liquid chromatography-tandem mass spectrometry

	Studies on the interactions between ginsenosides and liposome by equilibrium dialysis combined with ultrahigh performance liquid chromatography-tandem mass spectrometry
	Hou GY ; Niu J ; Song FR ; Liu ZQ ; Liu SY
刊名	journal of chromatography b-analytical technologies in the biomedical and life sciences
	2013
卷号	923 页码:1-7
关键词	EPITHELIAL CACO-2 CELLS NORTH-AMERICAN GINSENG PANAX-QUINQUEFOLIUS-L. ELECTROSPRAY-IONIZATION STRUCTURAL-ANALYSIS DRUG ABSORPTION DIFFERENTIATION PROTOPANAXADIOL IDENTIFICATION PHARMACOLOGY
ISSN号	1570-0232
通讯作者	song fr
中文摘要	to study the interactions between components of panax ginseng and liposome biomembrane, we applied the equilibrium dialysis system combined with ultrahigh performance liquid chromatography-tandem mass spectrometry (uplc-ms/ms) approach to analyze and identify the bioactive components of ginseng. moreover, the effect of ph value has also been investigated on their interactions between the ginsenosides of ginseng extract and biomembrane. the result shows that seven kinds of ginsenosides have obvious interactions with biomembrane in comparison with the standards in terms of tandem mass spectrometry (ms/ms) data along with retention time, including four panaxadiol ginsenosides (rb-1, rb-2, rc, rd) and three panaxatriol ginsenosides (re, rf, rg(2)). the value of binding degree decreased with the increase of molecular weight. the sugar moieties which are attached to c-20 were the main factor affecting the binding degree of panaxadiol ginsenosides. the interactions between panaxadiol ginsenosides and biomembrane correlate to the type and number of sugar moieties in ginsenosides. the sugar moieties which are at c-6 and c-20 have been shown to influence the value of binding degree for panaxatriol ginsenosides. in addition, the ph value has been shown to have an impact on the interactions. overall, ginsenoside rd has a better absorption character among the seven ginsenosides. in the study, we have screened the potential bioactive components of ginseng in vitro using the equilibrium dialysis-uplc-ms/ms method, and then predicted the potential bioactivities of ginseng, which contribute to the investigation of the efficacy of ginseng. (c) 2013 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000317148400001
公开日期	2014-04-16
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49943]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Hou GY,Niu J,Song FR,et al. Studies on the interactions between ginsenosides and liposome by equilibrium dialysis combined with ultrahigh performance liquid chromatography-tandem mass spectrometry[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2013,923:1-7.
APA	Hou GY,Niu J,Song FR,Liu ZQ,&Liu SY.(2013).Studies on the interactions between ginsenosides and liposome by equilibrium dialysis combined with ultrahigh performance liquid chromatography-tandem mass spectrometry.journal of chromatography b-analytical technologies in the biomedical and life sciences,923,1-7.
MLA	Hou GY,et al."Studies on the interactions between ginsenosides and liposome by equilibrium dialysis combined with ultrahigh performance liquid chromatography-tandem mass spectrometry".journal of chromatography b-analytical technologies in the biomedical and life sciences 923(2013):1-7.