Screening of alpha-Glucosidase Inhibitors in Ginkgo Biloba Extract Using Ultrafiltration LC-ESI-MSn

	Screening of alpha-Glucosidase Inhibitors in Ginkgo Biloba Extract Using Ultrafiltration LC-ESI-MSn
	Liu Y ; Zhou H ; Liu S ; Liu ZQ
刊名	chemical journal of chinese universities-chinese
	2013
卷号	34 期号:4 页码:813-818
关键词	MASS-SPECTROMETRY COMBINATORIAL LIBRARIES FLAVONOID FRACTION DNA
ISSN号	0251-0790
通讯作者	liu zq
中文摘要	in vitro alpha-glucosidase inhibition assays and ultrafiltration liquid chromatography coupled to electrospray ionization tandem mass spectrometry(ultrafiltration lc-esi-msn) were combined to screen alpha-glucosidase inhibitors from ginkgo biloba extract. the enzyme inhibition studies were carried out spectrophotometrically in a 96-well microplate reader. using 4-nitrophenyl alpha-d-glucopyranoside(pnpg) as the substrate and acarbose as a positive control, alpha-glucosidase inhibitory activity of the five kinds of chinese herb extracts which rich in flavonoids were determined, the results showed that the ginkgo biloba extract has the strongest the alpha-glucosidase inhibitory activity. ultrafiltration lc-est-msn was used to screen and identify alpha-glucosidase inhibitors from ginkgo biloba extract. as a result, four compounds were identified as alpha-glucosidase inhibitors in the ginkgo biloba extract, and their structures were confirmed to be quercetin, apigenin, kaempferol and isorhamnetin by esi-ms.
收录类别	SCI收录期刊论文
语种	中文
WOS记录号	WOS:000317558400011
公开日期	2014-04-16
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49920]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Liu Y,Zhou H,Liu S,et al. Screening of alpha-Glucosidase Inhibitors in Ginkgo Biloba Extract Using Ultrafiltration LC-ESI-MSn[J]. chemical journal of chinese universities-chinese,2013,34(4):813-818.
APA	Liu Y,Zhou H,Liu S,&Liu ZQ.(2013).Screening of alpha-Glucosidase Inhibitors in Ginkgo Biloba Extract Using Ultrafiltration LC-ESI-MSn.chemical journal of chinese universities-chinese,34(4),813-818.
MLA	Liu Y,et al."Screening of alpha-Glucosidase Inhibitors in Ginkgo Biloba Extract Using Ultrafiltration LC-ESI-MSn".chemical journal of chinese universities-chinese 34.4(2013):813-818.