Validated LC-ESI-MS/MS Method for the Quantitation of Neopanaxadiol: a Novel Neuroprotective Agent from Panax ginseng and Its Application to a Pharmacokinetic Study in Rat Plasma

	Validated LC-ESI-MS/MS Method for the Quantitation of Neopanaxadiol: a Novel Neuroprotective Agent from Panax ginseng and Its Application to a Pharmacokinetic Study in Rat Plasma
	Geng C ; Liu JH ; Guan QX ; Zhang Y ; Meng Q ; Sun DY ; Sun YQ ; Wang JW ; Zhou YH ; Yin JY
刊名	chromatographia
	2013
卷号	76 期号:41892 页码:509-514
关键词	TANDEM MASS-SPECTROMETRY 20(S)-PROTOPANAXADIOL METABOLISM URINE CELLS RG1 RE
ISSN号	0009-5893
通讯作者	yin jy
中文摘要	neopanaxadiol (npd), a new panaxadiol first obtained from the acid hydrolysate of the total ginsenosides of panax ginseng c. a. meyer (araliaceae) in our laboratory, showed neuroprotective effect against glutamate-induced neurotoxicity in primary cultures of rat cortical cells (tao et al. chin chem lett 20:687-689, 2009). in this research, a sensitive and specifical method of ultra-performance liquid chromatography-electrospray mass spectrometry (uplc-esi-ms/ms) for the quantitative analysis of npd in rat plasma was developed. the npd and internal standard of panaxadiol were extracted from 200 mu l of rat plasma samples with methanol, then the samples were injected in an uplc (waters beh) with c18 column (50 mm x 2.1 mm i.d., 1.7 mu m) and eluted with the mobile phase consisting of methanol-water-formic acid (80:20:0.2, v/v/v). excellent linearity was found between 5 and 160 ng ml(-1) with a limit of quantitation of 4 ng ml(-1). intra- and inter-day precision values (rsd) of qc samples were both below 7 %. this study was successfully utilized for the pharmacokinetic profiles of npd in rats after oral administration and provided an experimental basis for the further pharmacology and clinical application of ginseng products.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000318168100009
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49884]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Geng C,Liu JH,Guan QX,et al. Validated LC-ESI-MS/MS Method for the Quantitation of Neopanaxadiol: a Novel Neuroprotective Agent from Panax ginseng and Its Application to a Pharmacokinetic Study in Rat Plasma[J]. chromatographia,2013,76(41892):509-514.
APA	Geng C.,Liu JH.,Guan QX.,Zhang Y.,Meng Q.,...&Yin JY.(2013).Validated LC-ESI-MS/MS Method for the Quantitation of Neopanaxadiol: a Novel Neuroprotective Agent from Panax ginseng and Its Application to a Pharmacokinetic Study in Rat Plasma.chromatographia,76(41892),509-514.
MLA	Geng C,et al."Validated LC-ESI-MS/MS Method for the Quantitation of Neopanaxadiol: a Novel Neuroprotective Agent from Panax ginseng and Its Application to a Pharmacokinetic Study in Rat Plasma".chromatographia 76.41892(2013):509-514.