Detecting Type 2 Diabetes-related Single Nucleotide Polymorphisms by Microarray

	Detecting Type 2 Diabetes-related Single Nucleotide Polymorphisms by Microarray
	Chen Y ; Liu GF ; Liu X ; Zhang GZ ; Wang ZX
刊名	chemical journal of chinese universities-chinese
	2013
卷号	34 期号:5 页码:1078-1083
关键词	DNA MICROARRAY ACID
ISSN号	0251-0790
通讯作者	zhang gz
中文摘要	a three-dimensional (3d) oligonucleotide microarray-based fluorescent assay was developed for screening specific oligonucleotide probes for type 2 diabetes detection. the 3d oligonucleotide microarray was fabricated on the home-made fourth-generation (g4) nh2-terminated poly(amidoamine) dendrimers (pam-am) modified substrate. assay for rs7903146 site of the type 2 diabetes related gene tcf7l2 was developed. the utility of this assay was demonstrated with an oligonucleotide library, a collection of 16 (8 pairs) oligonucleotides, and one specific oligonucleotide probe pair for type 2 diabetes was obtained. taking advantage of the high specificity of the probe pair, the allele frequency as low as 2% can be accurately determined.
收录类别	SCI收录期刊论文
语种	中文
WOS记录号	WOS:000319546600009
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49862]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Chen Y,Liu GF,Liu X,et al. Detecting Type 2 Diabetes-related Single Nucleotide Polymorphisms by Microarray[J]. chemical journal of chinese universities-chinese,2013,34(5):1078-1083.
APA	Chen Y,Liu GF,Liu X,Zhang GZ,&Wang ZX.(2013).Detecting Type 2 Diabetes-related Single Nucleotide Polymorphisms by Microarray.chemical journal of chinese universities-chinese,34(5),1078-1083.
MLA	Chen Y,et al."Detecting Type 2 Diabetes-related Single Nucleotide Polymorphisms by Microarray".chemical journal of chinese universities-chinese 34.5(2013):1078-1083.