Label-free detection of single-stranded DNA binding protein based on a cantilever array

	Label-free detection of single-stranded DNA binding protein based on a cantilever array
	Hou H ; Bai XJ ; Xing CY ; Lu BP ; Hao JH ; Ke X ; Gu NY ; Zhang BL ; Tang JL
刊名	talanta
	2013
卷号	109 页码:173-176
关键词	ESCHERICHIA-COLI SSB MODES ENTHALPY SENSORS DOMAIN
ISSN号	0039-9140
通讯作者	gu ny
中文摘要	we report a simple and sensitive method for label-free detection of single-stranded dna-binding protein (ssbp) based on an array of microfabricated cantilevers. the single-stranded dna (ssdna) was immobilized on the surface of the sensing cantilevers to detect ssbp, while the reference cantilevers were modified with 6-mercapto-1-hexanol to detect any unwanted cantilever deflection. the differential deflection signals that reveal specific ssbp-ssdna binding have been found to depend on the ssbp concentration. using the cantilever array sensor we can detect ssbp in the concentration range from 0.01 to 7 mu g ml(-1). other proteins, such as thrombin or bovine serum albumin induced no significant deflection of the cantilevers. our results show the potential for the application of cantilever array sensor system as a powerful tool to detect proteins with high sensitivity and specificity. (c) 2013 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000319089100024
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49856]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Hou H,Bai XJ,Xing CY,et al. Label-free detection of single-stranded DNA binding protein based on a cantilever array[J]. talanta,2013,109:173-176.
APA	Hou H.,Bai XJ.,Xing CY.,Lu BP.,Hao JH.,...&Tang JL.(2013).Label-free detection of single-stranded DNA binding protein based on a cantilever array.talanta,109,173-176.
MLA	Hou H,et al."Label-free detection of single-stranded DNA binding protein based on a cantilever array".talanta 109(2013):173-176.