A fluorescence turn-on method for real-time monitoring of protease activity based on the electron transfer between a fluorophore labeled oligonucleotide and cytochrome c

	A fluorescence turn-on method for real-time monitoring of protease activity based on the electron transfer between a fluorophore labeled oligonucleotide and cytochrome c
	Liao DL ; Li YX ; Chen J ; Yu C
刊名	analytica chimica acta
	2013
卷号	784 页码:72-76
ISSN号	0003-2670
通讯作者	yu c
中文摘要	a new continuous fluorescence turn-on assay for protease activity and inhibitor screening has been developed. a fluorophore labeled single stranded dna (fam-dna) and cytochrome c (cyt c) were employed. the fluorescence of the fam-dna was efficiently quenched when binding to cyt c, through the electron transfer between the fam fluorophore and the heme cofactor of cyt c. in the presence of a protease, such as trypsin, cyt c was digested into small peptide fragments. the fam-dna was released, which resulted in the recovery of the fam fluorescence. the rate of the cyt c digestion could be reduced via the addition of an inhibitor. as a result, reduced degree of the fluorescence recovery was obtained. the limit of detection of our assay is 1 nm trypsin and the ic50 values are 3.23 mu gml(-1) and 0.303 1.mu gml(-1) for the inhibitor from egg white and the inhibitor from soybean, respectively. our method could be used for the sensing of protease activity for various biochemical applications, and for the screening of protease inhibitors as drugs for the treatment of various related diseases. (c) 2013 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000320826100010
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49792]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Liao DL,Li YX,Chen J,et al. A fluorescence turn-on method for real-time monitoring of protease activity based on the electron transfer between a fluorophore labeled oligonucleotide and cytochrome c[J]. analytica chimica acta,2013,784:72-76.
APA	Liao DL,Li YX,Chen J,&Yu C.(2013).A fluorescence turn-on method for real-time monitoring of protease activity based on the electron transfer between a fluorophore labeled oligonucleotide and cytochrome c.analytica chimica acta,784,72-76.
MLA	Liao DL,et al."A fluorescence turn-on method for real-time monitoring of protease activity based on the electron transfer between a fluorophore labeled oligonucleotide and cytochrome c".analytica chimica acta 784(2013):72-76.