Sensing of a nucleic acid binding protein via a label-free perylene probe fluorescence recovery assay

	Sensing of a nucleic acid binding protein via a label-free perylene probe fluorescence recovery assay
	Liao DL ; Li WY ; Chen J ; Jiao HP ; Zhou HP ; Wang B ; Yu C
刊名	analytica chimica acta
	2013
卷号	797 页码:89-94
关键词	TURN-ON DETECTION DNA-BINDING C-JUN TRANSCRIPTION FACTORS MOLECULAR BEACONS INDUCTION APOPTOSIS TARGETS
ISSN号	0003-2670
通讯作者	yu c
中文摘要	a novel label-free fluorescence recovery assay for the sensing of a dna binding protein has been developed. a transcription factor c-jun protein, and a 21 base pair duplex dna containing the c-jun protein binding site (j-dna) were selected. j-dna was mixed with a cationic fluorescent perylene probe (compound 1), and induced aggregation of the probe. quenching of the probe's fluorescence was observed. however, when c-jun protein was mixed with the j-dna, c-jun bound to the duplex dna, which reduced the degree of the induced perylene probe aggregation, and a turn on fluorescence signal was observed. the recovered fluorescence intensity was directly related to the amount of c-jun added. the method is highly selective, six non-dna binding proteins and one randomly selected 21 base pair duplex dna (con-1) were tested. no noticeable compound 1 fluorescence recovery was observed. mutations were also introduced to the c-jun recognition sequence and much reduced fluorescence recovery was observed. our assay is label-free, convenient, inexpensive, and fast. it can be used in biomedical research such as high throughput screening of drugs targeted at dna-binding proteins. (c) 2013 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000324524700010
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49552]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Liao DL,Li WY,Chen J,et al. Sensing of a nucleic acid binding protein via a label-free perylene probe fluorescence recovery assay[J]. analytica chimica acta,2013,797:89-94.
APA	Liao DL.,Li WY.,Chen J.,Jiao HP.,Zhou HP.,...&Yu C.(2013).Sensing of a nucleic acid binding protein via a label-free perylene probe fluorescence recovery assay.analytica chimica acta,797,89-94.
MLA	Liao DL,et al."Sensing of a nucleic acid binding protein via a label-free perylene probe fluorescence recovery assay".analytica chimica acta 797(2013):89-94.