Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain

	Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain
	Qiao XF ; Tian J ; Chen P ; Wang C ; Ni JZ ; Liu Q
刊名	international journal of molecular sciences
	2013
卷号	14 期号:11 页码:22233-22245
关键词	NEURAL STEM-CELLS REGULATES NEUROGENESIS INCREASED EXPRESSION NEUROTROPHIC FACTOR ADULT NEUROGENESIS BINDING PROTEIN TRANSGENIC RAT DELTA-FOSB PROLIFERATION SELENIUM
ISSN号	1422-0067
通讯作者	liu q
中文摘要	selenium, an essential trace element for human health, mainly exerts its biological function through selenoproteins. selenoprotein m (selm) is one of the highly expressed selenoproteins in the brain, but its biological effect and molecular mechanism remain unclear. thus, the interactive protein of selm was investigated in this paper to guide further study. in order to avoid protein translational stop, the selenocysteine-encoding uga inside the open reading frame of selm was site-directly changed to the cysteine-encoding ugc to generate the selm mutant. meanwhile, its n terminal transmembrane signal peptide was also cut off. this truncated selm was used to screen a human fetal brain cdna library by the yeast two-hybrid system. a new interactive protein of selm was found to be galectin-1 (gal-1). this protein-protein interaction was further verified by the results of fluorescence resonance energy transfer techniques, glutathione s-transferase pull-down and co-immunoprecipitation assays. as gal-1 plays important roles in preventing neurodegeneration and promoting neuroprotection in the brain, the interaction between selm and gal-1 displays a new direction for studying the biological function of selm in the human brain.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000328624400064
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49494]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Qiao XF,Tian J,Chen P,et al. Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain[J]. international journal of molecular sciences,2013,14(11):22233-22245.
APA	Qiao XF,Tian J,Chen P,Wang C,Ni JZ,&Liu Q.(2013).Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain.international journal of molecular sciences,14(11),22233-22245.
MLA	Qiao XF,et al."Galectin-1 Is an Interactive Protein of Selenoprotein M in the Brain".international journal of molecular sciences 14.11(2013):22233-22245.