Doxorubicin-loaded amphiphilic polypeptide-based nanoparticles as an efficient drug delivery system for cancer therapy

	Doxorubicin-loaded amphiphilic polypeptide-based nanoparticles as an efficient drug delivery system for cancer therapy
	Lv SX ; Li MQ ; Tang ZH ; Song WT ; Sun H ; Liu HY ; Chen XS
刊名	acta biomaterialia
	2013
卷号	9 期号:12 页码:9330-9342
关键词	BLOCK-COPOLYMER MICELLES PHASE-I INTRACELLULAR RELEASE CELL UPTAKE PH CARRIERS PEG PACLITAXEL REDUCTION COMPLEX
ISSN号	1742-7061
通讯作者	liu hy
中文摘要	an amphiphilic anionic copolymer, methoxy poly(ethylene glycol)-b-poly(l-glutamic acid-co-l-phenylalanine) (mpeg-b-p(glu-co-phe)), with three functionalized domains, was synthesized and used as a nanovehicle for cationic anticancer drug doxorubicin hydrochloride (dox-hcl) delivery via electrostatic interactions for cancer treatment. the three domains displayed distinct functions: peg block chain for prolonged circulation; poly(phenylalanine) domain for stabilizing the nanoparticle construct through hydrophobic/aromatic interactions; and the poly(glutamic acid) domain for providing electrostatic interactions with the cationic drug to be loaded. the copolymer could self-assemble into micellar-type nanoparticles, and dox was successfully loaded into the interior of nanoparticles by simple mixing of dox-hcl and the copolymer in the aqueous phase. dox-loaded mpeg-b-p(glu-co-phe) nanoparticles (dox-np) had a superior drug-loading content (dlc) (21.7%), a high loading efficiency (almost 98%) and a ph-triggered release of dox. the size of dox-np was similar to 140 nm, as determined by dynamic light scattering measurements and transmission electron microscopy. in vitro assays showed that dox-np exhibited higher cell proliferation inhibition and higher cell uptake in a549 cell lines compared with free dox-hcl. maximum tolerated dose (mtd) studies showed that dox-np demonstrated an excellent safety profile with a significantly higher mtd (15 mg dox kg(-1)) than that of free dox.hcl (5 mg dox kg(-1)). the in vivo studies on the subcutaneous non-small cell lung cancer (a549) xenograft nude mice model confirmed that dox-np showed significant antitumor activity and reduced side effects, and then enhanced tumor accumulation as a result of the prolonged circulation in blood and the enhanced permeation and retention effect, compared with free dox, indicating its great potential for cancer therapy. (c) 2013 acta materialia inc. published by elsevier ltd. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000328592600008
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49425]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Lv SX,Li MQ,Tang ZH,et al. Doxorubicin-loaded amphiphilic polypeptide-based nanoparticles as an efficient drug delivery system for cancer therapy[J]. acta biomaterialia,2013,9(12):9330-9342.
APA	Lv SX.,Li MQ.,Tang ZH.,Song WT.,Sun H.,...&Chen XS.(2013).Doxorubicin-loaded amphiphilic polypeptide-based nanoparticles as an efficient drug delivery system for cancer therapy.acta biomaterialia,9(12),9330-9342.
MLA	Lv SX,et al."Doxorubicin-loaded amphiphilic polypeptide-based nanoparticles as an efficient drug delivery system for cancer therapy".acta biomaterialia 9.12(2013):9330-9342.