Biodegradation-inspired bioproduction of methylacetoin and 2-methyl-2,3-butanediol

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Biodegradation-inspired bioproduction of methylacetoin and 2-methyl-2,3-butanediol
	Jiang, Xinglin 1,2; Zhang, Haibo 1; Yang, Jianming 1; Zheng, Yanning 1,2; Feng, Dexin 1; Liu, Wei 1; Xu, Xin 1; Cao, Yujin 1; Zou, Huibin 1; Zhang, Rubin 1
刊名	SCIENTIFIC REPORTS
	2013-08-15
卷号	3 期号:3 页码:2445
英文摘要	Methylacetoin (3-hydroxy-3-methylbutan-2-one) and 2-methyl-2,3-butanediol are currently obtained exclusively via chemical synthesis. Here, we report, to the best of our knowledge, the first alternative route, using engineered Escherichia coli. The biological synthesis of methylacetoin was first accomplished by reversing its biodegradation, which involved modifying the enzyme complex involved, switching the reaction substrate, and coupling the process to an exothermic reaction. 2-Methyl-2,3-butanediol was then obtained by reducing methylacetoin by exploiting the substrate promiscuity of acetoin reductase. A complete biosynthetic pathway from renewable glucose and acetone was then established and optimized via in vivo enzyme screening and host metabolic engineering, which led to titers of 3.4 and 3.2 g l(-1) for methylacetoin and 2-methyl-2,3-butanediol, respectively. This work presents a biodegradation-inspired approach to creating new biosynthetic pathways for small molecules with no available natural biosynthetic pathway.
学科主题	生物基化学品
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	ESCHERICHIA-COLI ; ACETOIN ; METABOLISM ; DEHYDROGENASE ; KETONE
收录类别	SCI
语种	英语
WOS记录号	WOS:000323099700003
公开日期	2014-03-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1679]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Xinglin,Zhang, Haibo,Yang, Jianming,et al. Biodegradation-inspired bioproduction of methylacetoin and 2-methyl-2,3-butanediol[J]. SCIENTIFIC REPORTS,2013,3(3):2445.
APA	Jiang, Xinglin.,Zhang, Haibo.,Yang, Jianming.,Zheng, Yanning.,Feng, Dexin.,...&Xian, Mo.(2013).Biodegradation-inspired bioproduction of methylacetoin and 2-methyl-2,3-butanediol.SCIENTIFIC REPORTS,3(3),2445.
MLA	Jiang, Xinglin,et al."Biodegradation-inspired bioproduction of methylacetoin and 2-methyl-2,3-butanediol".SCIENTIFIC REPORTS 3.3(2013):2445.