Yeast Surface Displaying Glucose Oxidase as Whole-Cell Biocatalyst: Construction, Characterization, and Its Electrochemical Glucose Sensing Application

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 生物传感技术团队

	Yeast Surface Displaying Glucose Oxidase as Whole-Cell Biocatalyst: Construction, Characterization, and Its Electrochemical Glucose Sensing Application
	Wang, Hongwei 1,2,3; Lang, Qiaolin 1,2; Li, Liang 1,2; Liang, Bo 1,2; Tang, Xiangjiang 1,2; Kong, Lingrang 3; Mascini, Marco 4; Liu, Aihua 1,2
刊名	ANALYTICAL CHEMISTRY
	2013-06-18
卷号	85 期号:12 页码:6107-6112
英文摘要	The display of glucose oxidase (GOx) on yeast cell surface using a-agglutinin as an anchor motif was successfully developed. Both the immunochemical analysis and enzymatic assay showed that active GOx was efficiently expressed and translocated on the cell surface. Compared with conventional GOx, the yeast cell surface that displayed GOx (GOx-yeast) demonstrated excellent enzyme properties, such as good stability within a wide pH range (pH 3.5-11.5), good therrnostability (retaining over 94.8% enzyme activity at 52 degrees C and 84.2% enzyme activity at 56 degrees C), and high D-glucose specificity. In addition, direct electrochemistry was achieved at a GOx-yeast/multiwalled-carbon-nanotube modified electrode, suggesting that the host cell of yeast did not have any adverse effect on the electrocatalytic property of the recombinant GOx. Thus, a novel electrochemical glucose biosensor based on this GOx-yeast was developed. The as-prepared biosensor was linear with the concentration of D-glucose within the range of 0.1-14 mM and a low detection limit of 0.05 mM (signal-to-noise ratio of S/N = 3). Moreover, the as-prepared biosensor is stable, specific, reproducible, simple, and cost-effective, which can be applicable for real sample detection. The proposed strategy to construct robust GOx-yeast may be applied to explore other oxidase-displaying-system-based whole-cell biocatalysts, which can find broad potential application in biosensors,, bioenergy, and industrial catalysis.
学科主题	生物传感器技术
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	ASPERGILLUS-NIGER ; SACCHAROMYCES-CEREVISIAE ; ELECTRON-TRANSFER ; DEHYDROGENASE ; EXPRESSION ; XYLOSE ; BIOSENSORS ; BACTERIA ; GENE ; IMMOBILIZATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000320749200069
公开日期	2014-03-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1692]
专题	青岛生物能源与过程研究所_生物传感技术团队
作者单位	1.Chinese Acad Sci, Lab Biosensing, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China 3.Shandong Agr Univ, Coll Agron, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 4.Univ Florence, Dipartimento Chim, I-50019 Sesto Fiorentino, Italy
推荐引用方式 GB/T 7714	Wang, Hongwei,Lang, Qiaolin,Li, Liang,et al. Yeast Surface Displaying Glucose Oxidase as Whole-Cell Biocatalyst: Construction, Characterization, and Its Electrochemical Glucose Sensing Application[J]. ANALYTICAL CHEMISTRY,2013,85(12):6107-6112.
APA	Wang, Hongwei.,Lang, Qiaolin.,Li, Liang.,Liang, Bo.,Tang, Xiangjiang.,...&Liu, Aihua.(2013).Yeast Surface Displaying Glucose Oxidase as Whole-Cell Biocatalyst: Construction, Characterization, and Its Electrochemical Glucose Sensing Application.ANALYTICAL CHEMISTRY,85(12),6107-6112.
MLA	Wang, Hongwei,et al."Yeast Surface Displaying Glucose Oxidase as Whole-Cell Biocatalyst: Construction, Characterization, and Its Electrochemical Glucose Sensing Application".ANALYTICAL CHEMISTRY 85.12(2013):6107-6112.