Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物多样性与资源保护研究中心 > 期刊论文

	Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China
	He, Dekui 1; Tang, Weixing 1; Li, Chunhua 1,2; Chen, Yifeng 1
刊名	CONSERVATION GENETICS RESOURCES
	2013-12-01
卷号	5 期号:4 页码:895-897
关键词	Schizopygopsis Schizothoracinae Enriched genomic library Population genetics Tibet
ISSN号	1877-7252
通讯作者	Chen, YF (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China.
中文摘要	Eighteen polymorphic microsatellite primers were developed for Schizopygopsis younghusbandi using an enrichment genomic library and evaluated for PCR using the genomic DNA of 29 individuals from the Lhasa River in Tibet. Eighteen of 70 loci amplified reliable bands revealed a useful degree of polymorphism with a typical multi-banded pattern of polyploid species. The total number of bands per locus ranged from 3 to 30. Average A (i) and H (o) were 3.563 and 0.916, respectively. The heterozygosity expected varied from 0.595 to 0.946 (RCeS) and from 0.580 to 0.923 (RCdS), respectively. These microsatellite primers would be useful in population genetics studies for this species and related species of genus Schizopygopsis.
英文摘要	Eighteen polymorphic microsatellite primers were developed for Schizopygopsis younghusbandi using an enrichment genomic library and evaluated for PCR using the genomic DNA of 29 individuals from the Lhasa River in Tibet. Eighteen of 70 loci amplified reliable bands revealed a useful degree of polymorphism with a typical multi-banded pattern of polyploid species. The total number of bands per locus ranged from 3 to 30. Average A (i) and H (o) were 3.563 and 0.916, respectively. The heterozygosity expected varied from 0.595 to 0.946 (RCeS) and from 0.580 to 0.923 (RCdS), respectively. These microsatellite primers would be useful in population genetics studies for this species and related species of genus Schizopygopsis.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]	Biodiversity & Conservation ; Genetics & Heredity
关键词[WOS]	SCHIZOTHORACINE FISHES TELEOSTEI ; MOLECULAR PHYLOGENY ; STRATEGIES ; SEQUENCE ; PROGRAM ; PLATEAU ; RIVER
收录类别	SCI
资助信息	NSFC [41030208, 31071900]; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences
语种	英语
WOS记录号	WOS:000326387800002
公开日期	2014-01-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19756]
专题	水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	He, Dekui,Tang, Weixing,Li, Chunhua,et al. Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China[J]. CONSERVATION GENETICS RESOURCES,2013,5(4):895-897.
APA	He, Dekui,Tang, Weixing,Li, Chunhua,&Chen, Yifeng.(2013).Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China.CONSERVATION GENETICS RESOURCES,5(4),895-897.
MLA	He, Dekui,et al."Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China".CONSERVATION GENETICS RESOURCES 5.4(2013):895-897.