Trans location dynamics of tRNA-mRNA in the ribosome

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	Trans location dynamics of tRNA-mRNA in the ribosome
	Xie, P
刊名	BIOPHYSICAL CHEMISTRY
	2013
卷号	180 页码:22
关键词	Ribosome Translocation Hybrid state Translation tRNA-mRNA
ISSN号	0301-4622
通讯作者	Xie, P (reprint author), Chinese Acad Sci, Inst Phys, Key Lab Soft Matter Phys, Beijing 100190, Peoples R China.
中文摘要	Translocation of tRNA-mRNA complex in the ribosome is an essential step in the elongation cycle of protein synthesis. However, some important issues concerning the molecular mechanism of the tRNA-mRNA translocation catalyzed by EF-G.GTP or by EF-G.GDPNP remain controversial. For example, can EF-G.GTP selectively bind to the hybrid pretranslocation state or bind to both the non-rotated pretranslocation and the hybrid pretranslocation states? Does the greater potency of EF-G in the presence of GTP rather than GDPNP in facilitating translocation derive from the effects on transition from the classical non-rotated to hybrid state (the first step of the translocation) or on transition from the hybrid to posttranslocation state (the second step)? Here, based on our proposed model, we study theoretically the dynamics of the tRNA-mRNA translocation through the ribosome catalyzed by EF-G.GTP and by EF-G.GDPNP. By comparing our theoretical results with the available experimental data, we show that EF-G.GTP can also bind to the classical non-rotated pretranslocation state and the greater potency of GTP hydrolysis in facilitating translocation of tRNA-mRNA complex derives from its effects on the second step of the translocation process. (C) 2013 Elsevier B.V. All rights reserved.
语种	英语
公开日期	2014-01-17
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/57617]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Xie, P. Trans location dynamics of tRNA-mRNA in the ribosome[J]. BIOPHYSICAL CHEMISTRY,2013,180:22.
APA	Xie, P.(2013).Trans location dynamics of tRNA-mRNA in the ribosome.BIOPHYSICAL CHEMISTRY,180,22.
MLA	Xie, P."Trans location dynamics of tRNA-mRNA in the ribosome".BIOPHYSICAL CHEMISTRY 180(2013):22.