Quantitative interpretation of gold nanoparticle-based bioassays designed for detection of immunocomplex formation

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	Quantitative interpretation of gold nanoparticle-based bioassays designed for detection of immunocomplex formation
	Zhou, Y ; Xu, H ; Dahlin, AB ; Vallkil, J ; Borrebaeck, CAK ; Wingren, C ; Liedberg, B ; Hook, F
刊名	BIOINTERPHASES
	2007
卷号	2 期号:1 页码:6
ISSN号	1559-4106
中文摘要	The authors present in this paper how the extended Mie theory can be used to translate not only end-point data but also temporal variations of extinction peak-position changes, Delta lambda(peak)(t), into absolute mass uptake, Gamma(t), upon biomacromolecule binding to localized surface plasmon resonance (SPR) active nanoparticles (NPs). The theoretical analysis is applied on a novel sensor template composed of a three-layer surface architecture based on (i) a self-assembled monolayer of HS(CH2)(15)COOH, (ii) a 1:1 mixture of biotinylated and pure poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG), and (iii) NeutrAvidin. Assisted by independent estimations of the thickness of the three-layer architecture using quartz crystal microbalance with dissipation (QCM-D) monitoring, excellent agreement with parallel mass-uptake estimations using planar SPR is obtained. Furthermore, unspecific binding of serum to PLL-g-PEG was shown to be below the detection limit, making the surface architecture ideally suited for label-free detection of immunoreactions. To ensure that the immunocomplex formation occurred within the limited sensing depth (similar to 10 nm) of the NPs, a compact model system composed of a biotinylated human recombinant single-chain antibody fragment (empty set similar to 2 nm) directed against cholera toxin was selected. By tracking changes in the centroid (center of mass) of the extinction peak, rather than the actual peak position, signal-to-noise levels and long-term stability upon cholera toxin detection are demonstrated to be competitive with results obtained using conventional SPR and state-of-the-art QCM-D data. (C) 2007 American Vacuum Society.
收录类别	SCI
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/51626]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Zhou, Y,Xu, H,Dahlin, AB,et al. Quantitative interpretation of gold nanoparticle-based bioassays designed for detection of immunocomplex formation[J]. BIOINTERPHASES,2007,2(1):6.
APA	Zhou, Y.,Xu, H.,Dahlin, AB.,Vallkil, J.,Borrebaeck, CAK.,...&Hook, F.(2007).Quantitative interpretation of gold nanoparticle-based bioassays designed for detection of immunocomplex formation.BIOINTERPHASES,2(1),6.
MLA	Zhou, Y,et al."Quantitative interpretation of gold nanoparticle-based bioassays designed for detection of immunocomplex formation".BIOINTERPHASES 2.1(2007):6.