Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures

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	Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures
	Xu, YN ; Bazeille, N ; Ding, XY ; Lu, XM ; Wang, PY ; Bugnard, E ; Grondin, V ; Dou, SX ; Xi, XG
刊名	NUCLEIC ACIDS RESEARCH
	2012
卷号	40 期号:19 页码:9802
关键词	BLOOMS-SYNDROME HELICASE SYNDROME GENE-PRODUCT WERNER-SYNDROME PROTEIN SINGLE-STRANDED-DNA RECQ HELICASES HEXAMERIC HELICASES TOPOISOMERASE III REPLICATION FORK ARGININE FINGER UVRD HELICASE
ISSN号	0305-1048
通讯作者	Dou, SX: Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China.
中文摘要	Bloom (BLM) syndrome is an autosomal recessive disorder characterized by an increased risk for many types of cancers. Previous studies have shown that BLM protein forms a hexameric ring structure, but its oligomeric form in DNA unwinding is still not well clarified. In this work, we have used dynamic light scattering and various stopped-flow assays to study the active form and kinetic mechanism of BLM in DNA unwinding. It was found that BLM multimers were dissociated upon ATP hydrolysis. Steady-state and single-turnover kinetic studies revealed that BLM helicase always unwound duplex DNA in the monomeric form under conditions of varying enzyme and ATP concentrations as well as 3'-ssDNA tail lengths, with no sign of oligomerization being discerned. Measurements of ATPase activity further indicated that BLM helicase might still function as monomers in resolving highly structured DNAs such as Holliday junctions and D-loops. These results shed new light on the underlying mechanism of BLM-mediated DNA unwinding and on the molecular and functional basis for the phenotype of heterozygous carriers of BLM syndrome.
收录类别	SCI
资助信息	National Natural Science Foundation of China [10834014]; National Basic Research Program of China [973 Program] [2009CB930704]; Centre National de la Recherche Scientifique (CNRS); CNRS
语种	英语
公开日期	2013-09-18
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/42391]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Xu, YN,Bazeille, N,Ding, XY,et al. Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures[J]. NUCLEIC ACIDS RESEARCH,2012,40(19):9802.
APA	Xu, YN.,Bazeille, N.,Ding, XY.,Lu, XM.,Wang, PY.,...&Xi, XG.(2012).Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures.NUCLEIC ACIDS RESEARCH,40(19),9802.
MLA	Xu, YN,et al."Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures".NUCLEIC ACIDS RESEARCH 40.19(2012):9802.