Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method

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	Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method
	Wang, X ; Yuan, K ; Lu, H ; Wen, J ; Lu, HB ; Jin, KJ ; Zhou, YL ; Yang, GZ ; Li, W ; Ruan, KC
刊名	JOURNAL OF APPLIED PHYSICS
	2010
卷号	107 期号:6
关键词	DNA PROBES FILMS
ISSN号	0021-8979
通讯作者	Yang, GZ: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China.
中文摘要	Hybridizations between labeled or label-free targets and corresponding 21-base oligonucleotide probes, concentrations of which range from 0.39 to 50 mu M, are detected by oblique-incidence reflectivity difference (OI-RD) method and fluorescence detection. The experimental results demonstrate that the OI-RD method can be utilized to not only distinguish whether the hybridization of oligonucleotides happened but also directly tell the different concentrations of the labeled and unlabeled oligonucleotides on the microarrays. The analysis with a classical three-layer model suggests that single-strand DNA tends to lie on epoxy-functionalized glass slide while the double-strand DNA prefers to have a tilted angle with respect to the slide in our experimental situation. The label-free detection of hybridization of oligonucleotides declares that OI-RD is a promising method for label-free and high-throughput detection of the biological microarrays.
收录类别	SCI
资助信息	National Basic Research Program in China [2007CB935700]
语种	英语
公开日期	2013-09-18
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/40534]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Wang, X,Yuan, K,Lu, H,et al. Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method[J]. JOURNAL OF APPLIED PHYSICS,2010,107(6).
APA	Wang, X.,Yuan, K.,Lu, H.,Wen, J.,Lu, HB.,...&Ruan, KC.(2010).Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method.JOURNAL OF APPLIED PHYSICS,107(6).
MLA	Wang, X,et al."Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method".JOURNAL OF APPLIED PHYSICS 107.6(2010).