Direct visualization of RecQ helicase-DNA interaction with fluorescence microscopy and atomic force microscopy

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	Direct visualization of RecQ helicase-DNA interaction with fluorescence microscopy and atomic force microscopy
	Liu, YY ; Wang, PY ; Dou, SX ; Wang, WC ; Xie, P ; Yin, HW ; Lin, Y ; Jiang, YX ; Fang, XH ; Xi, XG
刊名	SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
	2005
卷号	6 期号:7 页码:842
关键词	SYNDROME GENE-PRODUCT REPLICATION CHECKPOINT ASSAY PROTEIN UVRD
ISSN号	1468-6996
通讯作者	Wang, PY: Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing Natl Lab Condensed Matter Phys, POB 603, Beijing 100080, Peoples R China.
中文摘要	RecQ helicase-DNA interactions were directly visualized with fluorescence microscopy. DNA-RecQ complexes formed in binding and unwinding reaction were stretched onto the hydrophobic surface by molecular combing method. The complexes can be observed with fluorescence microscope because the DNA molecules were labeled with dye molecules of YOYO-1. The DNA binding and unwinding activity of RecQ helicase leads to reduced lengths of the observed DNA molecules. More direct observations with atomic force microscopy were also made. It was seen that RecQ is mainly monomeric both in solution and after binding to DNA. (c) 2005 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
公开日期	2013-09-17
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/36085]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Liu, YY,Wang, PY,Dou, SX,et al. Direct visualization of RecQ helicase-DNA interaction with fluorescence microscopy and atomic force microscopy[J]. SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS,2005,6(7):842.
APA	Liu, YY.,Wang, PY.,Dou, SX.,Wang, WC.,Xie, P.,...&Xi, XG.(2005).Direct visualization of RecQ helicase-DNA interaction with fluorescence microscopy and atomic force microscopy.SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS,6(7),842.
MLA	Liu, YY,et al."Direct visualization of RecQ helicase-DNA interaction with fluorescence microscopy and atomic force microscopy".SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS 6.7(2005):842.