Circadian KaiC Phosphorylation: A Multi-Layer Network

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	Circadian KaiC Phosphorylation: A Multi-Layer Network
	Li, CX ; Chen, XF ; Wang, PY ; Wang, WC
刊名	PLOS COMPUTATIONAL BIOLOGY
	2009
卷号	5 期号:11
关键词	GENE-EXPRESSION IN-VITRO SYNECHOCOCCUS-ELONGATUS CLOCK SYSTEM CYANOBACTERIA PROTEIN OSCILLATION MECHANISM MODEL DYNAMICS
ISSN号	1553-734X
通讯作者	Li, CX: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Lab Soft Matter Phys, POB 603, Beijing 100080, Peoples R China.
中文摘要	Circadian KaiC phosphorylation in cyanobacteria reconstituted in vitro recently initiates a series of studies experimentally and theoretically to explore its mechanism. In this paper, we report a dynamic diversity in hexameric KaiC phosphoforms using a multi-layer reaction network based on the nonequivalence of the dual phosphorylation sites (S431 and T432) in each KaiC subunit. These diverse oscillatory profiles can generate a kaleidoscopic phase modulation pattern probably responsible for the genome-wide transcription rhythms directly and/or indirectly in cyanobacteria. Particularly, our model reveals that a single KaiC hexamer is an energy-based, phosphorylation-dependent and self-regulated circadian oscillator modulated by KaiA and KaiB. We suggest that T432 is the main regulator for the oscillation amplitude, while S431 is the major phase regulator. S431 and T432 coordinately control the phosphorylation period. Robustness of the Kai network was examined by mixing samples in different phases, and varying protein concentrations and temperature. Similar results were obtained regardless of the deterministic or stochastic method employed. Therefore, the dynamic diversities and robustness of Kai oscillator make it a qualified core pacemaker that controls the cellular processes in cyanobacteria pervasively and accurately.
收录类别	SCI
资助信息	National Natural Science Foundation of China [10834014, 10674173, 30770517]; National Basic Research Program of China [2009CB930704]
语种	英语
公开日期	2013-09-17
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/34739]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Li, CX,Chen, XF,Wang, PY,et al. Circadian KaiC Phosphorylation: A Multi-Layer Network[J]. PLOS COMPUTATIONAL BIOLOGY,2009,5(11).
APA	Li, CX,Chen, XF,Wang, PY,&Wang, WC.(2009).Circadian KaiC Phosphorylation: A Multi-Layer Network.PLOS COMPUTATIONAL BIOLOGY,5(11).
MLA	Li, CX,et al."Circadian KaiC Phosphorylation: A Multi-Layer Network".PLOS COMPUTATIONAL BIOLOGY 5.11(2009).