A modified model for translocation events of processive nucleotide and repeat additions by the recombinant telomerase

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	A modified model for translocation events of processive nucleotide and repeat additions by the recombinant telomerase
	Xie, P
刊名	BIOPHYSICAL CHEMISTRY
	2010
卷号	153 期号:1 页码:83
关键词	TEMPLATE RNA COMPONENT TETRAHYMENA TELOMERASE REVERSE-TRANSCRIPTASE SECONDARY STRUCTURE CATALYTIC SUBUNIT CRYSTAL-STRUCTURE BINDING DOMAIN DNA-POLYMERASE STEM-LOOP PROTEIN
ISSN号	0301-4622
通讯作者	Xie, P: Chinese Acad Sci, Inst Phys, Key Lab Soft Matter Phys, Beijing 100190, Peoples R China.
中文摘要	Telomerase is a unique reverse transcriptase that extends the single-stranded 3' overhangs of telomeres by copying a short template sequence within the integral RNA component of the enzyme. It shows processive nucleotide and repeat addition activities, which are realized via two types of movements: translocation of the DNA:RNA hybrid away from the active site following each nucleotide addition and translocation of the 3' end of the DNA primer relative to the RNA template after each round of repeat synthesis. Here, a model is presented to describe these two types of translocation events by the recombinant Tetrahymena telomerase, via the modification of the model that has been proposed recently. Using the present model, the dynamics of the dissociation of the DNA primer from the telomerase and the dynamics of the disruption of the DNA:RNA hybrid and then repositioning of the product 3' end to the beginning of the template are studied quantitatively. Their effects on the repeat addition processivity are theoretically studied. The theoretical results are in agreement with the available experimental data. (C) 2010 Elsevier B.V. All rights reserved.
收录类别	SCI
资助信息	National Natural Science Foundation of China [10974248]
语种	英语
公开日期	2013-09-17
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/32996]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Xie, P. A modified model for translocation events of processive nucleotide and repeat additions by the recombinant telomerase[J]. BIOPHYSICAL CHEMISTRY,2010,153(1):83.
APA	Xie, P.(2010).A modified model for translocation events of processive nucleotide and repeat additions by the recombinant telomerase.BIOPHYSICAL CHEMISTRY,153(1),83.
MLA	Xie, P."A modified model for translocation events of processive nucleotide and repeat additions by the recombinant telomerase".BIOPHYSICAL CHEMISTRY 153.1(2010):83.