Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province

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	Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province
	Zhang, Dequan 1; Yang, Jing2 ; Jiang, Bei 1
刊名	BIOCHEMICAL SYSTEMATICS AND ECOLOGY
	2013-10-01
卷号	50 页码:448-451
关键词	Astragalus camptodontus Medicinal plant Microsatellite FIASCO Genetic diversity
ISSN号	0305-1978
通讯作者	Jiang,B (reprint author),Dali Univ,Coll Pharm & Chem,Dali 671000,Yunnan,Peoples R China.,northjiang@yahoo.com
英文摘要	Astragalus camptodontus is a traditional medicinal plant in Yunnan province, and it is used as one of substitutes for the Chinese medicine Astragali radix, namely "Huangqi". Twenty-three microsatellite loci were developed from its nuclear genome using a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Two wild populations collected from Northwest Yunnan were used to assess polymorphism of each locus. The number of alleles per locus ranged from 2 to 5 with a mean of 4.2. Values for Ho and HE ranged from 0.042 to 0.875 and from 0.254 to 0.793, with average of 0.403 and 0.616, respectively. These polymorphic loci will facilitate further studies on genetic diversity, population structure and gene flow of A. camptodontus and its allied species. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
关键词[WOS]	MARKERS
收录类别	SCI
资助信息	National Natural Science Foundation of China [31170313];Research Fund for the Large-scale Scientific Facilities of the Chinese Academy of Sciences [2009-LSF-GBOWS-01]
语种	英语
WOS记录号	WOS:000325597600065
公开日期	2014-01-13
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/17446]
专题	昆明植物研究所_中国西南野生生物种质资源库
作者单位	1.Dali Univ, Coll Pharm & Chem, Dali 671000, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Dequan,Yang, Jing,Jiang, Bei. Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2013,50:448-451.
APA	Zhang, Dequan,Yang, Jing,&Jiang, Bei.(2013).Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,50,448-451.
MLA	Zhang, Dequan,et al."Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 50(2013):448-451.