Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death

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	Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death
	Chen, Hongying 1,2; Osuna, Daniel 3; Colville, Louise 2; Lorenzo, Oscar 3; Graeber, Kai 4,5; Kuester, Helge 5; Leubner-Metzger, Gerhard 4; Kranner, Ilse 2
刊名	PLOS ONE
	2013-10-29
卷号	8 期号:10
关键词	PROTEIN S-GLUTATHIONYLATION SIGNAL-REGULATING KINASE-1 ARABIDOPSIS-THALIANA BAX INHIBITOR-1 DIFFERENTIAL EXPRESSION LIPID-PEROXIDATION INDUCED APOPTOSIS MESSENGER-RNA NICOTIANA-BENTHAMIANA MICROARRAY ANALYSIS
ISSN号	1932-6203
通讯作者	Colville,L (reprint author),Kew,Royal Bot Gardens,Seed Conservat Dept,Ardingly,W Sussex,England.,l.colville@kew.org
英文摘要	Understanding of seed ageing, which leads to viability loss during storage, is vital for ex situ plant conservation and agriculture alike. Yet the potential for regulation at the transcriptional level has not been fully investigated. Here, we studied the relationship between seed viability, gene expression and glutathione redox status during artificial ageing of pea (Pisum sativum) seeds. Transcriptome-wide analysis using microarrays was complemented with qRT-PCR analysis of selected genes and a multilevel analysis of the antioxidant glutathione. Partial degradation of DNA and RNA occurred from the onset of artificial ageing at 60% RH and 50 degrees C, and transcriptome profiling showed that the expression of genes associated with programmed cell death, oxidative stress and protein ubiquitination were altered prior to any sign of viability loss. After 25 days of ageing viability started to decline in conjunction with progressively oxidising cellular conditions, as indicated by a shift of the glutathione redox state towards more positive values (>-190 mV). The unravelling of the molecular basis of seed ageing revealed that transcriptome reprogramming is a key component of the ageing process, which influences the progression of programmed cell death and decline in antioxidant capacity that ultimately lead to seed viability loss.
学科主题	Science & Technology - Other Topics
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	PROTEIN S-GLUTATHIONYLATION ; SIGNAL-REGULATING KINASE-1 ; ARABIDOPSIS-THALIANA ; BAX INHIBITOR-1 ; DIFFERENTIAL EXPRESSION ; LIPID-PEROXIDATION ; INDUCED APOPTOSIS ; MESSENGER-RNA ; NICOTIANA-BENTHAMIANA ; MICROARRAY ANALYSIS
收录类别	SCI
资助信息	Spanish Ministerio de Educacion y Ciencia;Junta de Castilla y Leon [BIO2011-26940, CSD2007-00057, SA048A10-2];German Research Foundation [DFGLe720/ 7];Chinese Academy of Sciences [KSCX2-EW-J-24, Y3221411W1];Millennium Commission;Wellcome Trust;Orange Plc.;Defra
语种	英语
WOS记录号	WOS:000326270700090
公开日期	2014-01-13
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/17439]
专题	昆明植物研究所_中国西南野生生物种质资源库
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming, Peoples R China 2.Kew, Royal Bot Gardens, Seed Conservat Dept, Ardingly, W Sussex, England 3.Univ Salamanca, Fac Biol, Ctr Hispano Luso Invest Agr CIALE, Dept Fisiol Vegetal, E-37008 Salamanca, Spain 4.Univ Freiburg, Fac Biol, Inst Biol 2, D-79106 Freiburg, Germany 5.Leibniz Univ Hannover, Inst Plant Genet, Unit Plant Genom 4, D-30167 Hannover, Germany
推荐引用方式 GB/T 7714	Chen, Hongying,Osuna, Daniel,Colville, Louise,et al. Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death[J]. PLOS ONE,2013,8(10).
APA	Chen, Hongying.,Osuna, Daniel.,Colville, Louise.,Lorenzo, Oscar.,Graeber, Kai.,...&Kranner, Ilse.(2013).Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death.PLOS ONE,8(10).
MLA	Chen, Hongying,et al."Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death".PLOS ONE 8.10(2013).