DMD-based LED-illumination Super-resolution and optical sectioning microscopy

CORC > 西安光学精密机械研究所 > 中国科学院西安光学精密机械研究所 > 瞬态光学技术国家重点实验室

	DMD-based LED-illumination Super-resolution and optical sectioning microscopy
	Dan, Dan1 ; Lei, Ming1 ; Yao, Baoli1 ; Wang, Wen 2,3; Winterhalder, Martin 4; Zumbusch, Andreas 4; Qi, Yujiao 1; Xia, Liang 2,3; Yan, Shaohui 1; Yang, Yanlong1
刊名	scientific reports
	2013-01-23
卷号	3 期号:0 页码:1116
ISSN号	2045-2322
通讯作者	雷铭
产权排序	1
合作状况	国际
英文摘要	super-resolution three-dimensional (3d) optical microscopy has incomparable advantages over other high-resolution microscopic technologies, such as electron microscopy and atomic force microscopy, in the study of biological molecules, pathways and events in live cells and tissues. we present a novel approach of structured illumination microscopy (sim) by using a digital micromirror device (dmd) for fringe projection and a low-coherence led light for illumination. the lateral resolution of 90 nm and the optical sectioning depth of 120 mm were achieved. the maximum acquisition speed for 3d imaging in the optical sectioning mode was 1.63107 pixels/second, which was mainly limited by the sensitivity and speed of the ccd camera. in contrast to other sim techniques, the dmd-based led-illumination sim is cost-effective, ease of multi-wavelength switchable and speckle-noise-free. the 2d super-resolution and 3d optical sectioning modalities can be easily switched and applied to either fluorescent or non-fluorescent specimens.
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	digital micromirror device ; spatial light-modulator ; structured-illumination ; fluorescence microscopy ; reconstruction microscopy ; resolution limit ; nanoscopy ; cells
收录类别	SCI
语种	英语
WOS记录号	WOS:000314091000009
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.opt.ac.cn/handle/181661/20945]
专题	西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位	1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China 2.Fourth Mil Med Univ, Dept Anat, Xian 710032, Peoples R China 3.Fourth Mil Med Univ, KK Leung Brain Res Ctr, Xian 710032, Peoples R China 4.Univ Konstanz, Dept Chem, D-78457 Constance, Germany
推荐引用方式 GB/T 7714	Dan, Dan,Lei, Ming,Yao, Baoli,et al. DMD-based LED-illumination Super-resolution and optical sectioning microscopy[J]. scientific reports,2013,3(0):1116.
APA	Dan, Dan.,Lei, Ming.,Yao, Baoli.,Wang, Wen.,Winterhalder, Martin.,...&Zhao, Wei.(2013).DMD-based LED-illumination Super-resolution and optical sectioning microscopy.scientific reports,3(0),1116.
MLA	Dan, Dan,et al."DMD-based LED-illumination Super-resolution and optical sectioning microscopy".scientific reports 3.0(2013):1116.