Identification of a Novel Gig2 Gene Family Specific to Non-Amniote Vertebrates

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	Identification of a Novel Gig2 Gene Family Specific to Non-Amniote Vertebrates
	Zhang, Yi-Bing; Liu, Ting-Kai; Jiang, Jun; Shi, Jun; Liu, Ying; Li, Shun; Gui, Jian-Fang
刊名	PLOS ONE
	2013-04-04
卷号	8 期号:4 页码:e60588
关键词	DEPENDENT PROTEIN-KINASE ATLANTIC SALMON GENOME DUPLICATION ANTIVIRAL RESPONSE TELEOST FISH CELL-LINE EVOLUTION RNA VIRUS POLY(ADP-RIBOSE)
ISSN号	1932-6203
通讯作者	Zhang, YB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
中文摘要	Gig2 (grass carp reovirus (GCRV)-induced gene 2) is first identified as a novel fish interferon (IFN)-stimulated gene (ISG). Overexpression of a zebrafish Gig2 gene can protect cultured fish cells from virus infection. In the present study, we identify a novel gene family that is comprised of genes homologous to the previously characterized Gig2. EST/GSS search and in silico cloning identify 190 Gig2 homologous genes in 51 vertebrate species ranged from lampreys to amphibians. Further large-scale search of vertebrate and invertebrate genome databases indicate that Gig2 gene family is specific to non-amniotes including lampreys, sharks/rays, ray-finned fishes and amphibians. Phylogenetic analysis and synteny analysis reveal lineage-specific expansion of Gig2 gene family and also provide valuable evidence for the fish-specific genome duplication (FSGD) hypothesis. Although Gig2 family proteins exhibit no significant sequence similarity to any known proteins, a typical Gig2 protein appears to consist of two conserved parts: an N-terminus that bears very low homology to the catalytic domains of poly(ADP-ribose) polymerases (PARPs), and a novel C-terminal domain that is unique to this gene family. Expression profiling of zebrafish Gig2 family genes shows that some duplicate pairs have diverged in function via acquisition of novel spatial and/or temporal expression under stresses. The specificity of this gene family to non-amniotes might contribute to a large extent to distinct physiology in non-amniote vertebrates.
英文摘要	Gig2 (grass carp reovirus (GCRV)-induced gene 2) is first identified as a novel fish interferon (IFN)-stimulated gene (ISG). Overexpression of a zebrafish Gig2 gene can protect cultured fish cells from virus infection. In the present study, we identify a novel gene family that is comprised of genes homologous to the previously characterized Gig2. EST/GSS search and in silico cloning identify 190 Gig2 homologous genes in 51 vertebrate species ranged from lampreys to amphibians. Further large-scale search of vertebrate and invertebrate genome databases indicate that Gig2 gene family is specific to non-amniotes including lampreys, sharks/rays, ray-finned fishes and amphibians. Phylogenetic analysis and synteny analysis reveal lineage-specific expansion of Gig2 gene family and also provide valuable evidence for the fish-specific genome duplication (FSGD) hypothesis. Although Gig2 family proteins exhibit no significant sequence similarity to any known proteins, a typical Gig2 protein appears to consist of two conserved parts: an N-terminus that bears very low homology to the catalytic domains of poly(ADP-ribose) polymerases (PARPs), and a novel C-terminal domain that is unique to this gene family. Expression profiling of zebrafish Gig2 family genes shows that some duplicate pairs have diverged in function via acquisition of novel spatial and/or temporal expression under stresses. The specificity of this gene family to non-amniotes might contribute to a large extent to distinct physiology in non-amniote vertebrates.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	DEPENDENT PROTEIN-KINASE ; ATLANTIC SALMON ; GENOME DUPLICATION ; ANTIVIRAL RESPONSE ; TELEOST FISH ; CELL-LINE ; EVOLUTION ; RNA ; VIRUS ; POLY(ADP-RIBOSE)
收录类别	SCI
资助信息	973 National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31272690, 31172435]
语种	英语
WOS记录号	WOS:000319108100056
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19587]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yi-Bing,Liu, Ting-Kai,Jiang, Jun,et al. Identification of a Novel Gig2 Gene Family Specific to Non-Amniote Vertebrates[J]. PLOS ONE,2013,8(4):e60588.
APA	Zhang, Yi-Bing.,Liu, Ting-Kai.,Jiang, Jun.,Shi, Jun.,Liu, Ying.,...&Gui, Jian-Fang.(2013).Identification of a Novel Gig2 Gene Family Specific to Non-Amniote Vertebrates.PLOS ONE,8(4),e60588.
MLA	Zhang, Yi-Bing,et al."Identification of a Novel Gig2 Gene Family Specific to Non-Amniote Vertebrates".PLOS ONE 8.4(2013):e60588.