Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri

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	Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri
	Zhao, Li Juan 1,2; Lu, Jin Fang 2; Nie, P.2,3; Li, Ai Hua 2; Xiong, Bang Xi 1; Xie, Hai Xia 2
刊名	VETERINARY MICROBIOLOGY
	2013-09-27
卷号	166 期号:1-2 页码:233-241
关键词	Plasmid EseH EseI EscD Virulence Edwardsiella ictaluri
ISSN号	0378-1135
通讯作者	Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
中文摘要	Native plasmids pEI1 and pEI2 were detected in Edwardsiella ictaluri HSN-1 isolated from diseased yellow catfish (Pelteobagrus fulvidraco). EseH encoded by pEI1 and other two proteins, EseI and EscD, encoded by pEI2, were found with homology to type III secretion system (T3SS) proteins. To investigate their roles in pathogenesis, the native plasmids were cured based on plasmid incompatibility by introducing a Kan positive and SacB negative selection marker into gene spacer of the native plasmids. Mutants with the deletion of the target genes were obtained by reverse PCR and self-ligation, and all mutants were examined for their virulence effect in yellow catfish. Compared with the HSN-1 strain, the two mutants Delta eseH and Delta eseI were attenuated, while mutant Delta escD had increased virulence with higher Competitive Index (CI) value. The adherence and invasion assays on fish EPC cells indicated that Delta eseH and Delta eseI had decreased ability in adherence. Using E. tarda as surrogate, EseH and EseI were detected in culture supernatants, but EscD was not, with the secretion of EseH depending on T3SS. In addition, EseH and EseI were found translocated into host cells, and by means of subcellular fractionation, EseH was localized in membrane fraction of ZF4 cells, and EseI in the cytosol fraction. Hence, the role of these three genes in adherence, invasion and cellular replication was revealed from the pathogenic bacterium E. ictaluri. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要	Native plasmids pEI1 and pEI2 were detected in Edwardsiella ictaluri HSN-1 isolated from diseased yellow catfish (Pelteobagrus fulvidraco). EseH encoded by pEI1 and other two proteins, EseI and EscD, encoded by pEI2, were found with homology to type III secretion system (T3SS) proteins. To investigate their roles in pathogenesis, the native plasmids were cured based on plasmid incompatibility by introducing a Kan positive and SacB negative selection marker into gene spacer of the native plasmids. Mutants with the deletion of the target genes were obtained by reverse PCR and self-ligation, and all mutants were examined for their virulence effect in yellow catfish. Compared with the HSN-1 strain, the two mutants Delta eseH and Delta eseI were attenuated, while mutant Delta escD had increased virulence with higher Competitive Index (CI) value. The adherence and invasion assays on fish EPC cells indicated that Delta eseH and Delta eseI had decreased ability in adherence. Using E. tarda as surrogate, EseH and EseI were detected in culture supernatants, but EscD was not, with the secretion of EseH depending on T3SS. In addition, EseH and EseI were found translocated into host cells, and by means of subcellular fractionation, EseH was localized in membrane fraction of ZF4 cells, and EseI in the cytosol fraction. Hence, the role of these three genes in adherence, invasion and cellular replication was revealed from the pathogenic bacterium E. ictaluri. (C) 2013 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology ; Veterinary Sciences
研究领域[WOS]	Microbiology ; Veterinary Sciences
关键词[WOS]	III SECRETION SYSTEM ; CATFISH PELTEOBAGRUS-FULVIDRACO ; COMPLETE DNA-SEQUENCE ; EPC CELL-LINE ; ENTERIC SEPTICEMIA ; CHANNEL CATFISH ; BETA-LACTAMASE ; EFFECTOR ; TARDA ; TRANSLOCATION
收录类别	SCI
资助信息	National Natural Science Foundation of China (NSFC) [30972278, 31172442]; National Basic Research Program of China (973program) [2009CB118703]; Jimei University of Fujian Province
语种	英语
WOS记录号	WOS:000322848100026
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19574]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 3.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Li Juan,Lu, Jin Fang,Nie, P.,et al. Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri[J]. VETERINARY MICROBIOLOGY,2013,166(1-2):233-241.
APA	Zhao, Li Juan,Lu, Jin Fang,Nie, P.,Li, Ai Hua,Xiong, Bang Xi,&Xie, Hai Xia.(2013).Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri.VETERINARY MICROBIOLOGY,166(1-2),233-241.
MLA	Zhao, Li Juan,et al."Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri".VETERINARY MICROBIOLOGY 166.1-2(2013):233-241.