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	RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120
	Gao, Yuan; Gong, Yangmin; Xu, Xudong
刊名	FEMS MICROBIOLOGY LETTERS
	2013-07-01
卷号	344期号:2页码:130-137
关键词	RNase III activity aaftsH interference Anabaena
ISSN号	0378-1097
通讯作者	Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
中文摘要	RNase III is a group of dsRNA-specific ribonucleases that play important roles in RNA processing and metabolism. Alr0280 and All4107 in Anabaena sp. PCC7120 are highly similar to RNase III enzymes. In vitro, recombinant Alr0280 showed RNase III activity. In the same cyanobacterium, the expression of ftsH (FtsH protease) could be suppressed by overexpression of an artificial sense RNA (aaftsH) that was complementary to aftsH, an internal antisense RNA. The aaftsH interference was abolished by inactivation of alr0280, the RNase III-encoding gene, and restored by complementation of the mutant. A cyanobacterial homolog to hen1, an RNA methyltransferase gene, may also be required for the aaftsH interference. This is the first report of RNase III-dependent sense RNA interference in cyanobacteria, and the underlying mechanism remains to be elucidated.
英文摘要	RNase III is a group of dsRNA-specific ribonucleases that play important roles in RNA processing and metabolism. Alr0280 and All4107 in Anabaena sp. PCC7120 are highly similar to RNase III enzymes. In vitro, recombinant Alr0280 showed RNase III activity. In the same cyanobacterium, the expression of ftsH (FtsH protease) could be suppressed by overexpression of an artificial sense RNA (aaftsH) that was complementary to aftsH, an internal antisense RNA. The aaftsH interference was abolished by inactivation of alr0280, the RNase III-encoding gene, and restored by complementation of the mutant. A cyanobacterial homolog to hen1, an RNA methyltransferase gene, may also be required for the aaftsH interference. This is the first report of RNase III-dependent sense RNA interference in cyanobacteria, and the underlying mechanism remains to be elucidated.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	DOUBLE-STRANDED-RNA ; SP-STRAIN PCC-7120 ; COLI RIBONUCLEASE-III ; ANTISENSE-RNA ; GENE-EXPRESSION ; IN-VITRO ; HETEROCYST DIFFERENTIATION ; KINASE GENE ; MECHANISM ; DOMAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000320722600005
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19636]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yuan,Gong, Yangmin,Xu, Xudong. RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120[J]. FEMS MICROBIOLOGY LETTERS,2013,344(2):130-137.
APA	Gao, Yuan,Gong, Yangmin,&Xu, Xudong.(2013).RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120.FEMS MICROBIOLOGY LETTERS,344(2),130-137.
MLA	Gao, Yuan,et al."RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120".FEMS MICROBIOLOGY LETTERS 344.2(2013):130-137.

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