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	Tetrahymena-specific biomass evaluation with a DNA-based method
	Yan, Qingyun1; Yu, Yuhe1; Dai, Lili1,2
刊名	SCIENCEASIA
	2013-02-01
卷号	39期号:1页码:36-41
关键词	genomic DNA real-time quantitative PCR molecular methods
ISSN号	1513-1874
通讯作者	Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
中文摘要	Evaluating the biomass of a specific taxon or that of a certain nutritional level is especially important for understanding an ecosystem. Although various methods are available, there is still a lack of a universally accepted approach for taxa-specific biomass evaluation. Taking cultured unicellular Tetrahymena as an example, the present study is aimed at exploring and highlighting a DNA-based method for taxa-specific biomass estimation. Results indicated that the Tetrahymena DNA yield increased linearly with increasing number of Tetrahymena cells (p<0.001). The intra and inter real-time quantitative PCR (Q-PCR) assays were highly reproducible, and Q-PCR quantifying 18S rDNA could detect an equivalent quantity of a single cell or less. Tetrahymena-specific biomass then can be determined according to the rDNA copies quantified by Q-PCR (p < 0.002), considering both of species richness and evenness (indicated by the pooled cells of Tetrahymena species with different ratios). Observations of the present study together with some others suggest that it is possible to quantify a target micro-biomass in natural ambience via specifically-amplified genes.
英文摘要	Evaluating the biomass of a specific taxon or that of a certain nutritional level is especially important for understanding an ecosystem. Although various methods are available, there is still a lack of a universally accepted approach for taxa-specific biomass evaluation. Taking cultured unicellular Tetrahymena as an example, the present study is aimed at exploring and highlighting a DNA-based method for taxa-specific biomass estimation. Results indicated that the Tetrahymena DNA yield increased linearly with increasing number of Tetrahymena cells (p<0.001). The intra and inter real-time quantitative PCR (Q-PCR) assays were highly reproducible, and Q-PCR quantifying 18S rDNA could detect an equivalent quantity of a single cell or less. Tetrahymena-specific biomass then can be determined according to the rDNA copies quantified by Q-PCR (p < 0.002), considering both of species richness and evenness (indicated by the pooled cells of Tetrahymena species with different ratios). Observations of the present study together with some others suggest that it is possible to quantify a target micro-biomass in natural ambience via specifically-amplified genes.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	REAL-TIME PCR ; QUANTITATIVE PCR ; ENVIRONMENTAL-SAMPLES ; MOLECULAR ECOLOGY ; MICROBIAL BIOMASS ; BACTERIAL-DNA ; QUANTIFICATION ; GENE ; SOIL ; SEA
收录类别	SCI
资助信息	National Natural Science Foundation of China [31071896]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture [201007]; Youth Innovation Promotion Association, CAS [Y22Z07]
语种	英语
WOS记录号	WOS:000324222400005
公开日期	2014-01-03
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19562]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yan, Qingyun,Yu, Yuhe,Dai, Lili. Tetrahymena-specific biomass evaluation with a DNA-based method[J]. SCIENCEASIA,2013,39(1):36-41.
APA	Yan, Qingyun,Yu, Yuhe,&Dai, Lili.(2013).Tetrahymena-specific biomass evaluation with a DNA-based method.SCIENCEASIA,39(1),36-41.
MLA	Yan, Qingyun,et al."Tetrahymena-specific biomass evaluation with a DNA-based method".SCIENCEASIA 39.1(2013):36-41.

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