Why mammals more susceptible to the hepatotoxic microcystins than fish: evidences from plasma and albumin protein binding through equilibrium dialysis

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	Why mammals more susceptible to the hepatotoxic microcystins than fish: evidences from plasma and albumin protein binding through equilibrium dialysis
	Zhang, Wei 1,2; Liang, Gaodao 1; Wu, Laiyan 1; Tuo, Xun 1; Wang, Wenjing 1; Chen, Jun 1; Xie, Ping 1
刊名	ECOTOXICOLOGY
	2013-08-01
卷号	22 期号:6 页码:1012-1019
关键词	Microcystins Plasma protein Albumin Equilibrium dialysis
ISSN号	0963-9292
通讯作者	Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China.
中文摘要	To elucidate the interspecies variation of susceptibility to microcystins (MCs), fresh plasma and purified albumin from six kinds of mammals and fish were used in toxins-substances binding test. Protein contents in the test plasma were analyzed and the binding characteristics to MCs were compared. Two kinds of widely observed MCs, microcystin-LR (MC-LR) and microcystin-RR (MC-RR) were tested and data were collected through the method of equilibrium dialysis. It was found that total plasma protein and albumin content in mammals were nearly two times and four times higher than that in fish, respectively. In the test range of 0-100 mu g/mL, binding rates of fish plasma to MCs were considered significant lower (p < 0.01) than that of mammals. And human plasma demonstrated the highest binding rate in mammals. In all the test species, plasma protein binding rates of MC-RR were significantly higher than MC-LR (p < 0.01). Besides, binding profiles of albumin were acquired under the protein content of 0.67 mg/mL. Human serum albumin demonstrated the highest affinity to MCs throughout the six species and differences among the other five species were considered not significant (p > 0.05). From the view of protein binding, it is concluded that both the variation of plasma protein composition and albumin binding characteristic could influence the existing form of MCs in circulation, change MCs utilization, alter MCs half-life and further contribute to the difference of susceptibility between mammals and fish.
英文摘要	To elucidate the interspecies variation of susceptibility to microcystins (MCs), fresh plasma and purified albumin from six kinds of mammals and fish were used in toxins-substances binding test. Protein contents in the test plasma were analyzed and the binding characteristics to MCs were compared. Two kinds of widely observed MCs, microcystin-LR (MC-LR) and microcystin-RR (MC-RR) were tested and data were collected through the method of equilibrium dialysis. It was found that total plasma protein and albumin content in mammals were nearly two times and four times higher than that in fish, respectively. In the test range of 0-100 mu g/mL, binding rates of fish plasma to MCs were considered significant lower (p < 0.01) than that of mammals. And human plasma demonstrated the highest binding rate in mammals. In all the test species, plasma protein binding rates of MC-RR were significantly higher than MC-LR (p < 0.01). Besides, binding profiles of albumin were acquired under the protein content of 0.67 mg/mL. Human serum albumin demonstrated the highest affinity to MCs throughout the six species and differences among the other five species were considered not significant (p > 0.05). From the view of protein binding, it is concluded that both the variation of plasma protein composition and albumin binding characteristic could influence the existing form of MCs in circulation, change MCs utilization, alter MCs half-life and further contribute to the difference of susceptibility between mammals and fish.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Ecology ; Environmental Sciences ; Toxicology
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
关键词[WOS]	CYCLIC PEPTIDE TOXIN ; HUMAN SERUM-ALBUMIN ; CYPRINUS-CARPIO L ; BLUE-GREEN-ALGA ; INTRAPERITONEAL INJECTION ; TISSUE DISTRIBUTION ; ATLANTIC SALMON ; OCHRATOXIN-A ; IN-VIVO ; LR
收录类别	SCI
资助信息	State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ07]
语种	英语
WOS记录号	WOS:000322166100005
公开日期	2014-01-03
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19549]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wei,Liang, Gaodao,Wu, Laiyan,et al. Why mammals more susceptible to the hepatotoxic microcystins than fish: evidences from plasma and albumin protein binding through equilibrium dialysis[J]. ECOTOXICOLOGY,2013,22(6):1012-1019.
APA	Zhang, Wei.,Liang, Gaodao.,Wu, Laiyan.,Tuo, Xun.,Wang, Wenjing.,...&Xie, Ping.(2013).Why mammals more susceptible to the hepatotoxic microcystins than fish: evidences from plasma and albumin protein binding through equilibrium dialysis.ECOTOXICOLOGY,22(6),1012-1019.
MLA	Zhang, Wei,et al."Why mammals more susceptible to the hepatotoxic microcystins than fish: evidences from plasma and albumin protein binding through equilibrium dialysis".ECOTOXICOLOGY 22.6(2013):1012-1019.