Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling

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	Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling
	Ma, Xufa 1; Liu, Jing-Xia 2
刊名	PLOS ONE
	2013-05-22
卷号	8 期号:5 页码:e64576
关键词	HEMATOPOIETIC STEM-CELLS TRANSCRIPTION FACTOR SELF-RENEWAL ZEBRAFISH EMBRYOS DNA-BINDING DIFFERENTIATION GATA-1 SPECIFICATION ACTIVATION GENE
ISSN号	1932-6203
通讯作者	Liu, JX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China.
中文摘要	ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. Our previous studies showed that eaf factors mediated effective convergence and extension (C&E) movements and modulated mesoderm and neural patterning by regulating both non-canonical and canonical Wnt signaling in the early embryonic process. In this study, through knockdown of both eaf1 and eaf2 in embryos, we found that differentiation of primary erythroid cells was blocked, but hematopoietic precursor cells maintained in eafs morphants. Co-injection of c-myb-MO rescued the erythroid differentiation in eafs morphants, as indicated by the restored expression of the erythroid-specific gene, beta e3 globin. In addition, low dosage of c-myb effectively blocked the beta e3 globin expression in embryos, and did not affect the expression of markers of hematopoietic progenitor cells and other mesoderm, which was similar to the phenotypes we observed in eafs morphants. We also revealed that knockdown Wnt signaling by transiently inducing dn-Tcf in embryos at the bud stage down-regulated the increased c-myb to normal level and also restored beta e3 globin expression in eafs morphants. Our evidence points to a novel role for eaf factors in controlling erythroid cell fate by regulating c-Myb expression through canonic Wnt signaling.
英文摘要	ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. Our previous studies showed that eaf factors mediated effective convergence and extension (C&E) movements and modulated mesoderm and neural patterning by regulating both non-canonical and canonical Wnt signaling in the early embryonic process. In this study, through knockdown of both eaf1 and eaf2 in embryos, we found that differentiation of primary erythroid cells was blocked, but hematopoietic precursor cells maintained in eafs morphants. Co-injection of c-myb-MO rescued the erythroid differentiation in eafs morphants, as indicated by the restored expression of the erythroid-specific gene, beta e3 globin. In addition, low dosage of c-myb effectively blocked the beta e3 globin expression in embryos, and did not affect the expression of markers of hematopoietic progenitor cells and other mesoderm, which was similar to the phenotypes we observed in eafs morphants. We also revealed that knockdown Wnt signaling by transiently inducing dn-Tcf in embryos at the bud stage down-regulated the increased c-myb to normal level and also restored beta e3 globin expression in eafs morphants. Our evidence points to a novel role for eaf factors in controlling erythroid cell fate by regulating c-Myb expression through canonic Wnt signaling.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	HEMATOPOIETIC STEM-CELLS ; TRANSCRIPTION FACTOR ; SELF-RENEWAL ; ZEBRAFISH EMBRYOS ; DNA-BINDING ; DIFFERENTIATION ; GATA-1 ; SPECIFICATION ; ACTIVATION ; GENE
收录类别	SCI
资助信息	Chinese Academy of Sciences [KSCX2-EW-Q-12]
语种	英语
WOS记录号	WOS:000320362700156
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19615]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Ma, Xufa,Liu, Jing-Xia. Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling[J]. PLOS ONE,2013,8(5):e64576.
APA	Ma, Xufa,&Liu, Jing-Xia.(2013).Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling.PLOS ONE,8(5),e64576.
MLA	Ma, Xufa,et al."Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling".PLOS ONE 8.5(2013):e64576.