Endocrine disruption by di-(2-ethylhexyl)-phthalate in Chinese rare minnow (Gobiocypris rarus)

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	Endocrine disruption by di-(2-ethylhexyl)-phthalate in Chinese rare minnow (Gobiocypris rarus)
	Wang, Xiaofang 1,2; Yang, Yuanjin 3; Zhang, Liping 1; Ma, Yanbo 1; Han, Jian 1; Yang, Lihua 1; Zhou, Bingsheng 1
刊名	ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
	2013-08-01
卷号	32 期号:8 页码:1846-1854
关键词	Di-(2-ethylhexyl)-phthalate (DEHP) Hypothalamic-pituitary-gonadal (HPG) axis Sex hormones Endocrine disruption Chinese rare minnow
ISSN号	0730-7268
通讯作者	Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
中文摘要	Great concern has been raised over the potential impact of environmental contaminants on fish populations that inhabit the Three Gorge Reservoir. The present study investigated the endocrine-disrupting effects of di-(2-ethylhexyl)-phthalate (DEHP) on the Chinese rare minnow (Gobiocypris rarus), an endemic fish distributed in upstream waters in the Yangtze River. Adult rare minnow were exposed to environmentally relevant concentrations of DEHP (0 mu g/L, 3.6 mu g/L, 12.8 mu g/L, 39.4 mu g/L, and 117.6 mu g/L) for a 21-d period. Then, concentrations of sex hormones in the plasma and relative transcription of various associated genes were measured in the hypothalamic-pituitary-gonadal (HPG) axis and liver of the fish. Exposure to DEHP resulted in greater circulating concentrations of testosterone (T) and lower concentrations of estradiol (E2), which were accompanied by upregulation of Cyp17 mRNA and downregulation of Cyp19a mRNA in the gonads of females. In males, increases of T and E2 levels were consistent with upregulation of Cyp17 and Cyp19a in the gonads. Furthermore, the T/E2 ratio was increased in females but reduced in males. A significant increase in the levels of hepatic vitellogenin (VTG) gene transcription was observed in both females and males. The present study showed that waterborne exposure to DEHP altered plasma sex hormone levels and modulated gene transcription profiles of associated genes in the HPG axis and liver, occurring mostly at higher concentrations (>39.4 mu g/L), which suggests that environmental concentration of DEHP (5.4 mu g/L) alone might not disturb the endocrine system of the rare minnow in the TGR. Environ Toxicol Chem 2013;32:1846-1854. (c) 2013 SETAC
英文摘要	Great concern has been raised over the potential impact of environmental contaminants on fish populations that inhabit the Three Gorge Reservoir. The present study investigated the endocrine-disrupting effects of di-(2-ethylhexyl)-phthalate (DEHP) on the Chinese rare minnow (Gobiocypris rarus), an endemic fish distributed in upstream waters in the Yangtze River. Adult rare minnow were exposed to environmentally relevant concentrations of DEHP (0 mu g/L, 3.6 mu g/L, 12.8 mu g/L, 39.4 mu g/L, and 117.6 mu g/L) for a 21-d period. Then, concentrations of sex hormones in the plasma and relative transcription of various associated genes were measured in the hypothalamic-pituitary-gonadal (HPG) axis and liver of the fish. Exposure to DEHP resulted in greater circulating concentrations of testosterone (T) and lower concentrations of estradiol (E2), which were accompanied by upregulation of Cyp17 mRNA and downregulation of Cyp19a mRNA in the gonads of females. In males, increases of T and E2 levels were consistent with upregulation of Cyp17 and Cyp19a in the gonads. Furthermore, the T/E2 ratio was increased in females but reduced in males. A significant increase in the levels of hepatic vitellogenin (VTG) gene transcription was observed in both females and males. The present study showed that waterborne exposure to DEHP altered plasma sex hormone levels and modulated gene transcription profiles of associated genes in the HPG axis and liver, occurring mostly at higher concentrations (>39.4 mu g/L), which suggests that environmental concentration of DEHP (5.4 mu g/L) alone might not disturb the endocrine system of the rare minnow in the TGR. Environ Toxicol Chem 2013;32:1846-1854. (c) 2013 SETAC
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences ; Toxicology
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
关键词[WOS]	PHTHALIC-ACID ESTERS ; PITUITARY-GONADAL AXIS ; GENE-EXPRESSION ; PIMEPHALES-PROMELAS ; CYPRINUS-CARPIO ; FATHEAD MINNOWS ; BISPHENOL-A ; TOXICITY ; RECEPTOR ; RATS
收录类别	SCI
资助信息	Science and Technology Research Project of China Three Gorges Corporation [07011034]
语种	英语
WOS记录号	WOS:000321549200022
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19595]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.China Three Gorges Corp, Inst Chinese Sturgeon Res, Yichang, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaofang,Yang, Yuanjin,Zhang, Liping,et al. Endocrine disruption by di-(2-ethylhexyl)-phthalate in Chinese rare minnow (Gobiocypris rarus)[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2013,32(8):1846-1854.
APA	Wang, Xiaofang.,Yang, Yuanjin.,Zhang, Liping.,Ma, Yanbo.,Han, Jian.,...&Zhou, Bingsheng.(2013).Endocrine disruption by di-(2-ethylhexyl)-phthalate in Chinese rare minnow (Gobiocypris rarus).ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,32(8),1846-1854.
MLA	Wang, Xiaofang,et al."Endocrine disruption by di-(2-ethylhexyl)-phthalate in Chinese rare minnow (Gobiocypris rarus)".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 32.8(2013):1846-1854.