Isolation and Purification of Antibacterial Peptides from Mussel

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 研究所（批量导入）

	Isolation and Purification of Antibacterial Peptides from Mussel
	Zhe LI; Bin ZHANG; Ming GU; Changhai WANG
刊名	Agricultural Biotechnology
	2012
期号	02 页码:20-23
关键词	Antibacterial peptides Mussel Isolation Purification
中文摘要	[Objective] This study aimed to extract antibacterial peptides from mussel. [Method] Blue mussels were used as raw materials for direct extraction of antibacterial peptides by using 0.5% acetic acid, and the antibacterial peptides were isolated and purified by Sephacryl S-100 polyacrylamide gel chromatography. The fractions were collected for measurement of antibacterial activity and minimum inhibitory concentration for a variety of bacterial species by filter paper diffusion assay. Molecular weight of the antibacterial peptides was determined by SDS-PAGE. Variation of antibacterial activity of antibacterial peptides was measured at 100 ℃ under conditions of different processing time and different pH. [Result] The 0.5% acetic acid was used for crude extraction of antibacterial peptides as extraction solution and led to relatively high extraction efficiency. By using Sephacryl S-100, the antibacterial peptides could be purified as a single substance. The isolated and purified antibacterial peptides of mussel had relatively strong antibacterial properties with molecular weight of 5 908, showing heat-resistance acid-alkaline resistance. [Conclusion] This study laid the theoretical foundation for large-scale production of antibacterial peptides.
收录类别	SCI
公开日期	2013-12-24
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6984]
专题	过程工程研究所_研究所（批量导入）
推荐引用方式 GB/T 7714	Zhe LI,Bin ZHANG,Ming GU,et al. Isolation and Purification of Antibacterial Peptides from Mussel[J]. Agricultural Biotechnology,2012(02):20-23.
APA	Zhe LI,Bin ZHANG,Ming GU,&Changhai WANG.(2012).Isolation and Purification of Antibacterial Peptides from Mussel.Agricultural Biotechnology(02),20-23.
MLA	Zhe LI,et al."Isolation and Purification of Antibacterial Peptides from Mussel".Agricultural Biotechnology .02(2012):20-23.