Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification | |
Jiang Yanjun1,2; Wang Qi1; Wang Wenqin1; Zhou Liya1; Gao Jing1 | |
刊名 | CHINESE JOURNAL OF CATALYSIS
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2012-05-01 | |
卷号 | 33期号:5页码:857-862 |
关键词 | cross-linked enzyme aggregate biomimetic silicification lipase Candida sp 99-125 genipin immobilized enzyme |
ISSN号 | 0253-9837 |
其他题名 | Chin. J. Catal. |
中文摘要 | The hybrid biocatalyst was prepared by the combination of cross-linked enzyme aggregates (CLEAs) and biomimetic silicification, where the lipase Candida sp. 99-125 was used as model enzyme. The preparation conditions of lipase-CLEAs were optimized and the resulting activity of 771 U/g was obtained. Under the optimum conditions, 75% of the activity recovery was obtained. P/CLEAs was prepared by coprecipitation of lipase with polyethylene-imine (PEI), and the resulting activity was 897 U/g. The activity recovery of P/CLEAs was about 88%. The silica coating was formed on the outside surface of P/CLEAs via the induction of PEI. The resulting Coated-CLEAs showed high reusability. The stability of the Coated-CLEAs, including the resistance to trypsin and organic solvents, was also significantly improved. |
英文摘要 | The hybrid biocatalyst was prepared by the combination of cross-linked enzyme aggregates (CLEAs) and biomimetic silicification, where the lipase Candida sp. 99-125 was used as model enzyme. The preparation conditions of lipase-CLEAs were optimized and the resulting activity of 771 U/g was obtained. Under the optimum conditions, 75% of the activity recovery was obtained. P/CLEAs was prepared by coprecipitation of lipase with polyethylene-imine (PEI), and the resulting activity was 897 U/g. The activity recovery of P/CLEAs was about 88%. The silica coating was formed on the outside surface of P/CLEAs via the induction of PEI. The resulting Coated-CLEAs showed high reusability. The stability of the Coated-CLEAs, including the resistance to trypsin and organic solvents, was also significantly improved. |
WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
类目[WOS] | Chemistry, Applied ; Chemistry, Physical ; Engineering, Chemical |
研究领域[WOS] | Chemistry ; Engineering |
关键词[WOS] | STABILIZATION ; ACYLASE ; LINKING ; BIOCATALYST ; SUPPORTS ; SILICA |
收录类别 | SCI |
原文出处 | |
语种 | 英语 |
WOS记录号 | WOS:000304019200017 |
公开日期 | 2013-11-28 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://ir.ipe.ac.cn/handle/122111/6450] ![]() |
专题 | 过程工程研究所_研究所(批量导入) |
作者单位 | 1.Hebei Univ Technol, Sch Chem Engn, Tianjin 300130, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Jiang Yanjun,Wang Qi,Wang Wenqin,et al. Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification[J]. CHINESE JOURNAL OF CATALYSIS,2012,33(5):857-862. |
APA | Jiang Yanjun,Wang Qi,Wang Wenqin,Zhou Liya,&Gao Jing.(2012).Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification.CHINESE JOURNAL OF CATALYSIS,33(5),857-862. |
MLA | Jiang Yanjun,et al."Preparation of Immobilized Lipase through Combination of Cross-Linked Enzyme Aggregates and Biomimetic Silicification".CHINESE JOURNAL OF CATALYSIS 33.5(2012):857-862. |
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