Interaction between Reduced Glutathione and PEO-PPO-PEO Copolymers in Aqueous Solutions: Studied by H-1 NMR and Spin-Lattice Relaxation

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	Interaction between Reduced Glutathione and PEO-PPO-PEO Copolymers in Aqueous Solutions: Studied by H-1 NMR and Spin-Lattice Relaxation
	Jia, Lianwei 1,2; Guo, Chen 1; Yang, Liangrong 1; Xiang, Junfeng 3; Tang, Yalin 3; Liu, Huizhou 1
刊名	JOURNAL OF PHYSICAL CHEMISTRY B
	2011-03-17
卷号	115 期号:10 页码:2228-2233
关键词	magnetic-resonance-spectroscopy pluronic((r)) block-copolymers multidrug-resistance proteins spontaneous vesicle formation oxide) triblock copolymer drug-delivery affinity-chromatography nmr-spectroscopy micellization micelles
ISSN号	1520-6106
其他题名	J. Phys. Chem. B
中文摘要	In order to investigate the effect of PEO-PPO-PEO copolymers on the glutathione (GSH)/glutathione-S-transferase (GST) detoxification system, interaction between the copolymers and GSH is studied by NMR measurements. Selective rotating-frame nuclear Overhauser effect (ROE) experiment confirms that glutamyl (Glu) alpha-H of GSH has spatial contact with EO methylene protons. Spin-lattice relaxation times of GSH Glu alpha-H show a decrease when PEO-PPO-PEO copolymers are added, and the decrease is greater with copolymers possessing more EO units. Other protons of GSH show little change in the presence of the copolymers. The addition of GSH promotes the dehydration of PEO-PPO-PEO copolymers. This results from the breaking of hydrogen bonds between water and the polymers and the forming of hydrogen bonds between Glu alpha-carboxylate protons and oxygen atoms of EO units. The dissociation constant between GSH and P85 copolymer is determined by spin-lattice relaxation measurements, which shows the binding is Of low affinity and the two molecules are in fast dissociation kinetics. This study suggests that GSH transporting or utilizing systems may be affected by treatment of PEO-PPO-PEO copolymers.
英文摘要	In order to investigate the effect of PEO-PPO-PEO copolymers on the glutathione (GSH)/glutathione-S-transferase (GST) detoxification system, interaction between the copolymers and GSH is studied by NMR measurements. Selective rotating-frame nuclear Overhauser effect (ROE) experiment confirms that glutamyl (Glu) alpha-H of GSH has spatial contact with EO methylene protons. Spin-lattice relaxation times of GSH Glu alpha-H show a decrease when PEO-PPO-PEO copolymers are added, and the decrease is greater with copolymers possessing more EO units. Other protons of GSH show little change in the presence of the copolymers. The addition of GSH promotes the dehydration of PEO-PPO-PEO copolymers. This results from the breaking of hydrogen bonds between water and the polymers and the forming of hydrogen bonds between Glu alpha-carboxylate protons and oxygen atoms of EO units. The dissociation constant between GSH and P85 copolymer is determined by spin-lattice relaxation measurements, which shows the binding is Of low affinity and the two molecules are in fast dissociation kinetics. This study suggests that GSH transporting or utilizing systems may be affected by treatment of PEO-PPO-PEO copolymers.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Physical
研究领域[WOS]	Chemistry
关键词[WOS]	MAGNETIC-RESONANCE-SPECTROSCOPY ; PLURONIC((R)) BLOCK-COPOLYMERS ; MULTIDRUG-RESISTANCE PROTEINS ; SPONTANEOUS VESICLE FORMATION ; OXIDE) TRIBLOCK COPOLYMER ; DRUG-DELIVERY ; AFFINITY-CHROMATOGRAPHY ; NMR-SPECTROSCOPY ; MICELLIZATION ; MICELLES
收录类别	SCI
原文出处	://WOS:000288113300011
语种	英语
WOS记录号	WOS:000288113300011
公开日期	2013-11-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6377]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Jia, Lianwei,Guo, Chen,Yang, Liangrong,et al. Interaction between Reduced Glutathione and PEO-PPO-PEO Copolymers in Aqueous Solutions: Studied by H-1 NMR and Spin-Lattice Relaxation[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2011,115(10):2228-2233.
APA	Jia, Lianwei,Guo, Chen,Yang, Liangrong,Xiang, Junfeng,Tang, Yalin,&Liu, Huizhou.(2011).Interaction between Reduced Glutathione and PEO-PPO-PEO Copolymers in Aqueous Solutions: Studied by H-1 NMR and Spin-Lattice Relaxation.JOURNAL OF PHYSICAL CHEMISTRY B,115(10),2228-2233.
MLA	Jia, Lianwei,et al."Interaction between Reduced Glutathione and PEO-PPO-PEO Copolymers in Aqueous Solutions: Studied by H-1 NMR and Spin-Lattice Relaxation".JOURNAL OF PHYSICAL CHEMISTRY B 115.10(2011):2228-2233.