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	Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography
	Tan, Tianwei3; Su, Zhi-Guo2; Gu, Ming2; Xu, Jun3; Janson, Jan-Christer1
刊名	BIOTECHNOLOGY JOURNAL
	2010-05-01
卷号	5期号:5页码:505-510
关键词	Cross-linked agarose Green tea catechin HILIC Mixed-mode separation Polyphenol separation
ISSN号	1860-6768
其他题名	Biotechnol. J.
中文摘要	Following its market introduction in 1982, the cross-linked 12% agarose gel media Superose 12 has become widely known as a tool for size exclusion chromatography of proteins and other biological macromolecules. In this review it is shown that, when appropriate mobile phases are used, Superose possesses adsorption properties similar to that of traditional media for hydrophilic interaction liquid chromatography (HILIC). This is illustrated by the separation and purification of low molecular weight compounds such as polyphenols including active components of traditional Chinese medicinal herbs and green tea. Structural features of the cross-linked agarose that likely cause the observed adsorption effects are discussed aswell. These are identified as being primarily ether bonds acting as strong hydrogen bond acceptors as well as hydrophobic residues originating from the cross-linking reagents.
英文摘要	Following its market introduction in 1982, the cross-linked 12% agarose gel media Superose 12 has become widely known as a tool for size exclusion chromatography of proteins and other biological macromolecules. In this review it is shown that, when appropriate mobile phases are used, Superose possesses adsorption properties similar to that of traditional media for hydrophilic interaction liquid chromatography (HILIC). This is illustrated by the separation and purification of low molecular weight compounds such as polyphenols including active components of traditional Chinese medicinal herbs and green tea. Structural features of the cross-linked agarose that likely cause the observed adsorption effects are discussed aswell. These are identified as being primarily ether bonds acting as strong hydrogen bond acceptors as well as hydrophobic residues originating from the cross-linking reagents.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	BOND ADSORPTION CHROMATOGRAPHY ; ONE-STEP PURIFICATION ; 12-PERCENT AGAROSE ; GEL-FILTRATION ; MEDIA ; PARTICLES ; GALLATE
收录类别	SCI
原文出处	://WOS:000278461800011
语种	英语
WOS记录号	WOS:000278461800011
公开日期	2013-11-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6291]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Uppsala Univ, Uppsala Biomed Ctr, Dept Phys & Analyt Chem, SE-75123 Uppsala, Sweden 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Dept Bioengn, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Tan, Tianwei,Su, Zhi-Guo,Gu, Ming,et al. Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography[J]. BIOTECHNOLOGY JOURNAL,2010,5(5):505-510.
APA	Tan, Tianwei,Su, Zhi-Guo,Gu, Ming,Xu, Jun,&Janson, Jan-Christer.(2010).Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography.BIOTECHNOLOGY JOURNAL,5(5),505-510.
MLA	Tan, Tianwei,et al."Cross-linked agarose for separation of low molecular weight natural products in hydrophilic interaction liquid chromatography".BIOTECHNOLOGY JOURNAL 5.5(2010):505-510.

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