Computational fluid dynamics modeling of mass-transfer behavior in a bioreactor for hairy root culture. II. Analysis of ultrasound-intensified process

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	Computational fluid dynamics modeling of mass-transfer behavior in a bioreactor for hairy root culture. II. Analysis of ultrasound-intensified process
	Liu, Rui 1,2; Sun, Wei 3; Liu, Chun-Zhao 1,2
刊名	BIOTECHNOLOGY PROGRESS
	2011-11-01
卷号	27 期号:6 页码:1672-1679
关键词	ultrasound computational fluid dynamics oxygen mass transfer membrane permeability airlift bioreactor
ISSN号	8756-7938
其他题名	Biotechnol. Prog.
中文摘要	Recently, cichoric acid production from hairy roots of Echinacea purpurea was significantly improved by ultrasound stimulation in an airlift bioreactor. In this article, the possible mechanism on ultrasound-intensified hairy root culture of E. purpurea in the bioreactor was elucidated with the help of computational fluid dynamics (CFD) simulation, membrane permeability detection, dissolved oxygen concentration detection, confocal laser-scanning microscopy (LSM) observation, and phenylalanine ammonium lyase (PAL) activity analysis. The CFD model developed in Part I was used to simulate the hydrodynamics and oxygen mass transfer in hairy root bioreactor culture stimulated by ultrasound. A dynamic mesh model combined with a changing Schmidt number method was used for the simulation of the ultrasound field. Simulation results and experimental data illustrated that ultrasound intensified oxygen mass transfer in the hairy root clump, which subsequently stimulated root growth and cichoric acid biosynthesis. Ultrasound increased the hairy root membrane permeability, and a high root membrane permeability of 0.359 h(-1) was observed at the bottom region in the bioreactor. LSM observation showed that the change in the membrane permeability recovered to normal in the further culture after ultrasound stimulation. PAL activity in the hairy roots was stimulated by ultrasound increase and was correlated well to cichoric acid accumulation in the hairy roots of E. purpurea. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011
英文摘要	Recently, cichoric acid production from hairy roots of Echinacea purpurea was significantly improved by ultrasound stimulation in an airlift bioreactor. In this article, the possible mechanism on ultrasound-intensified hairy root culture of E. purpurea in the bioreactor was elucidated with the help of computational fluid dynamics (CFD) simulation, membrane permeability detection, dissolved oxygen concentration detection, confocal laser-scanning microscopy (LSM) observation, and phenylalanine ammonium lyase (PAL) activity analysis. The CFD model developed in Part I was used to simulate the hydrodynamics and oxygen mass transfer in hairy root bioreactor culture stimulated by ultrasound. A dynamic mesh model combined with a changing Schmidt number method was used for the simulation of the ultrasound field. Simulation results and experimental data illustrated that ultrasound intensified oxygen mass transfer in the hairy root clump, which subsequently stimulated root growth and cichoric acid biosynthesis. Ultrasound increased the hairy root membrane permeability, and a high root membrane permeability of 0.359 h(-1) was observed at the bottom region in the bioreactor. LSM observation showed that the change in the membrane permeability recovered to normal in the further culture after ultrasound stimulation. PAL activity in the hairy roots was stimulated by ultrasound increase and was correlated well to cichoric acid accumulation in the hairy roots of E. purpurea. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Food Science & Technology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Food Science & Technology
关键词[WOS]	CELL-SUSPENSION-CULTURES ; LOW-ENERGY ULTRASOUND ; OXYGEN REQUIREMENTS ; ENHANCEMENT ; DELIVERY ; SYSTEMS ; FERMENTATION ; HYDROLYSIS ; METABOLISM ; GROWTH
收录类别	SCI
原文出处	://WOS:000297551300018
语种	英语
WOS记录号	WOS:000297551300018
公开日期	2013-11-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6288]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Gen Hosp AF, Dept Informat, Beijing 100142, Peoples R China
推荐引用方式 GB/T 7714	Liu, Rui,Sun, Wei,Liu, Chun-Zhao. Computational fluid dynamics modeling of mass-transfer behavior in a bioreactor for hairy root culture. II. Analysis of ultrasound-intensified process[J]. BIOTECHNOLOGY PROGRESS,2011,27(6):1672-1679.
APA	Liu, Rui,Sun, Wei,&Liu, Chun-Zhao.(2011).Computational fluid dynamics modeling of mass-transfer behavior in a bioreactor for hairy root culture. II. Analysis of ultrasound-intensified process.BIOTECHNOLOGY PROGRESS,27(6),1672-1679.
MLA	Liu, Rui,et al."Computational fluid dynamics modeling of mass-transfer behavior in a bioreactor for hairy root culture. II. Analysis of ultrasound-intensified process".BIOTECHNOLOGY PROGRESS 27.6(2011):1672-1679.