Solid Phase Pegylation of Hemoglobin

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	Solid Phase Pegylation of Hemoglobin
	Suo, Xiaoyan; Zheng, Chunyang; Yu, Pengzhan; Lu, Xiuling; Ma, Guanghui; Su, Zhiguo
刊名	ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
	2009
卷号	37 期号:4 页码:147-155
关键词	solid phase polyethylene glycol hemoglobin red blood cell substitute
ISSN号	1073-1199
通讯作者	Su, ZG
英文摘要	A solid phase conjugation process was developed for attachment of polyethylene glycol to hemoglobin molecule. Bovine hemoglobin was loaded onto an ion exchange chromatography column and adsorbed by the solid medium. Succinimidyl carbonate mPEG was introduced in the mobile phase after the adsorption. Pegylation took place between the hemoglobin on the solid phase, and the pegylation reagent in the liquid phase. A further elution was carried out to separate the pegylated and the unpegylated protein. Analysis by HPSEC, SDS-PAGE, and MALLS demonstrated that the fractions eluted from the solid phase contained well-defined components. Pegylated hemoglobin with one PEG chain was obtained with the yield of 75%, in comparison to the yield of 30% in the liquid phase pegylation. The P50 values of the mono-pegylated hemoglobin, prepared with SC-mPEG 5kDa, 10kDa and 20kDa, were 19.97, 20.23 and 20.54 mmHg, which were much closer to the value of red blood cells than that of pegylated hemoglobin prepared with the conventional method.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Biotechnology & Applied Microbiology ; Engineering ; Materials Science
关键词[WOS]	BOVINE SERUM-ALBUMIN ; POLYETHYLENE-GLYCOL ; COVALENT ATTACHMENT ; BLOOD ; PROTEINS ; SURFACE
收录类别	SCI
语种	英语
WOS记录号	WOS:000267835500001
公开日期	2013-12-11
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6768]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Suo, Xiaoyan,Zheng, Chunyang,Yu, Pengzhan,et al. Solid Phase Pegylation of Hemoglobin[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2009,37(4):147-155.
APA	Suo, Xiaoyan,Zheng, Chunyang,Yu, Pengzhan,Lu, Xiuling,Ma, Guanghui,&Su, Zhiguo.(2009).Solid Phase Pegylation of Hemoglobin.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,37(4),147-155.
MLA	Suo, Xiaoyan,et al."Solid Phase Pegylation of Hemoglobin".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 37.4(2009):147-155.