A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation, purification and biochemical characterization

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 生化工程国家重点实验室

	A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation, purification and biochemical characterization
	Suo, Xiaoyan 1,2; Lu, Xiuling 1; Hu, Tao 1; Ma, Guanghui 1; Su, Zhiguo 1
刊名	BIOTECHNOLOGY LETTERS
	2009-08-01
卷号	31 期号:8 页码:1191-1196
关键词	Human serum albumin PEGylation solid-phase Staphylokinase
ISSN号	0141-5492
通讯作者	Hu, T
英文摘要	A solid-phase adsorption method was developed to circumvent the disadvantage of the conventional liquid-phase PEGylation, i.e. the heterogeneity of the PEGylated products. The model proteins, human serum albumin (HSA) and staphylokinase (SAK), were adsorbed on the ion exchange chromatography media, followed by PEGylation with succinimidyl carbonate (SC)-mPEG5K and salt elution. Since PEGylation with SC-PEG5K alters the positive charge of the proteins, Q-Sepharose Big Beads and DEAE Sepharose Fast Flow were used for adsorption of HSA and SAK, respectively. Size exclusion chromatography and SDS-PAGE studies demonstrated that solid-phase PEGylation of proteins generate monoPEGylated proteins with the yield of 35-47%. Circular dichroism and intrinsic fluorescence studies showed that solid-phase PEGylation led to little conformational change of the proteins. Solid-phase PEGylation resulted in 35% loss in the biological activity of SAK, which is lower than the liquid-phase PEGylation (70%).
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	HEMOGLOBIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000268002100010
公开日期	2013-12-05
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6609]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Suo, Xiaoyan,Lu, Xiuling,Hu, Tao,et al. A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation, purification and biochemical characterization[J]. BIOTECHNOLOGY LETTERS,2009,31(8):1191-1196.
APA	Suo, Xiaoyan,Lu, Xiuling,Hu, Tao,Ma, Guanghui,&Su, Zhiguo.(2009).A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation, purification and biochemical characterization.BIOTECHNOLOGY LETTERS,31(8),1191-1196.
MLA	Suo, Xiaoyan,et al."A solid-phase adsorption method for PEGylation of human serum albumin and staphylokinase: preparation, purification and biochemical characterization".BIOTECHNOLOGY LETTERS 31.8(2009):1191-1196.