Identification of avermectin-high-producing strains by high-throughput screening methods

	Identification of avermectin-high-producing strains by high-throughput screening methods
	Gao, H ; Liu, M ; Zhou, XL ; Liu, JT ; Zhuo, Y ; Gou, ZX ; Xu, B ; Zhang, WQ ; Liu, XY ; Luo, AQ ; Zheng, CS ; Chen, XP ; Zhang, LX
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2010
卷号	85 期号:4 页码:1219-1225
关键词	Avermectin High-throughput screening Strain improvement Miniaturized production
ISSN号	0175-7598
通讯作者	Zhanglixin@im.ac.cn
中文摘要	Avermectins produced by Streptomyces avermitilis are potent against a broad spectrum of nematode and arthropod parasites with low-level side effects on the host organisms. This study was designed to investigate a high-throughput screening strategy for the efficient identification of avermectin high-yield strains. The production protocol was miniaturized in 96 deep-well microplates. UV absorbance at 245 nm was used to monitor avermectin production. A good correlation between fermentation results in both 96 deep-well microplates and conventional Erlenmeyer flasks was observed. With this protocol, the production of avermectins was determined in less than 10 min for a full plate without compromising accuracy. The high-yield strain selected through this protocol was also tested in 360 m(3) batch fermentation with 1.6-fold improved outcome. Thus, the development of this protocol is expected to accelerate the selection of superior avermectin-producing strains.
学科主题	Biotechnology & Applied Microbiology
收录类别	SCI
原文出处	SPRINGER
WOS记录号	WOS:000273554900040
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/5440]
专题	南海海洋研究所_海洋生物
推荐引用方式 GB/T 7714	Gao, H,Liu, M,Zhou, XL,et al. Identification of avermectin-high-producing strains by high-throughput screening methods[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,85(4):1219-1225.
APA	Gao, H.,Liu, M.,Zhou, XL.,Liu, JT.,Zhuo, Y.,...&Zhang, LX.(2010).Identification of avermectin-high-producing strains by high-throughput screening methods.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,85(4),1219-1225.
MLA	Gao, H,et al."Identification of avermectin-high-producing strains by high-throughput screening methods".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 85.4(2010):1219-1225.