Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment

	Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment
	Hu, YF ; Fu, CZ ; Huang, YP ; Yin, YS ; Cheng, G ; Lei, F ; Lu, N ; Li, J ; Ashforth, EJ ; Zhang, LX ; Zhu, BL
刊名	FEMS MICROBIOLOGY ECOLOGY
	2010
卷号	72 期号:2 页码:228-237
关键词	metagenomic library marine sediment lipolytic lipase esterase fosmid clone
ISSN号	0168-6496
通讯作者	zhubaoli@im.ac.cn
中文摘要	Metagenomic cloning is a powerful tool for the discovery of novel genes and biocatalysts from environmental microorganisms. Based on activity screening of a marine sediment microbial metagenomic library, a total of 19 fosmid clones showing lipolytic activity were identified. After subcloning, 15 different lipolytic genes were obtained; their encoded proteins showed 32-68% amino acid identity with proteins in the database. Multiple sequence alignment and phylogenetic tree analysis demonstrated that most of these predicted proteins are new members of known families of bacterial lipolytic enzymes. However, two proteins, FLS18C and FLS18D, could not be assigned to any known family, thus probably representing a novel family of the bacterial lipolytic enzyme. The activity assay results indicated that most of these lipolytic enzymes showed optimum temperature for hydrolysis at 40-50 degrees C with p-nitrophenol butyrate as a substrate. The lipolytic gene fls18D was overexpressed, and the resulting protein FLS18D was characterized as an alkaline esterase. Furthermore, the whole sequence of fosmid pFL18 containing FLS18C and FLS18D was shotgun sequenced, and a total of 26 ORFs on it were analyzed and annotated.
学科主题	Microbiology
收录类别	SCI
原文出处	WILEY-BLACKWELL PUBLISHING, INC
WOS记录号	WOS:000276490200007
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/5432]
专题	南海海洋研究所_海洋生物
推荐引用方式 GB/T 7714	Hu, YF,Fu, CZ,Huang, YP,et al. Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment[J]. FEMS MICROBIOLOGY ECOLOGY,2010,72(2):228-237.
APA	Hu, YF.,Fu, CZ.,Huang, YP.,Yin, YS.,Cheng, G.,...&Zhu, BL.(2010).Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment.FEMS MICROBIOLOGY ECOLOGY,72(2),228-237.
MLA	Hu, YF,et al."Novel lipolytic genes from the microbial metagenomic library of the South China Sea marine sediment".FEMS MICROBIOLOGY ECOLOGY 72.2(2010):228-237.