Dye Surface Coating Enables Visible Light Activation of TiO2 Nanoparticles Leading to Degradation of Neighboring Biological Structures

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 宁波所知识产出

	Dye Surface Coating Enables Visible Light Activation of TiO2 Nanoparticles Leading to Degradation of Neighboring Biological Structures
	Eric Brown ; Jay Blatnik,1,† Lanette Luebke,1,† Stephanie Simonet,1 Megan Nelson,1 Race Price,1 Rachael Leek,1 Leyong Zeng,2 Aiguo Wu,2 and Eric Brown1,*
刊名	Microscopy and Microanalysis
	2012-09-12
期号	18 页码:134—142
通讯作者	Eric Brown
合作状况	李雨桐
中文摘要	Biologically and chemically modified nanoparticles are gaining much attention as a new tool in cancer detection and treatment. Herein, we demonstrate that an alizarin red S ~ARS! dye coating on TiO2 nanoparticles enables visible light activation of the nanoparticles leading to degradation of neighboring biological structures through localized production of reactive oxygen species. Successful coating of nanoparticles with dye is demonstrated through sedimentation, spectrophotometry, and gel electrophoresis techniques. Using gel electrophoresis, we demonstrate that visible light activation of dye-TiO2 nanoparticles leads to degradation of plasmid DNA in vitro. Alterations in integrity and distribution of nuclear membrane associated proteins were detected via fluorescence confocal microscopy in HeLa cells exposed to perinuclear localized ARS-TiO2 nanoparticles that were photoactivated with visible light. This study expands upon previous studies that indicated dye coatings on TiO2 nanoparticles can serve to enhance imaging, by clearly showing that dye coatings on TiO2 nanoparticles can also enhance the photoreactivity of TiO2 nanoparticles by allowing visible light activation. The findings of our study suggest a therapeutic application of dye-coated TiO2 nanoparticles in cancer research; however, at the same time they may reveal limitations on the use of dye assisted visualization of TiO2 nanoparticles in live-cell imaging.
学科主题	材料科学
原文出处	SCI收录
公开日期	2013-12-16
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/9695]
专题	宁波材料技术与工程研究所_宁波所知识产出
推荐引用方式 GB/T 7714	Eric Brown,Jay Blatnik,1,† Lanette Luebke,1,† Stephanie Simonet,1 Megan Nelson,1 Race Price,1 Rachael Leek,1 Leyong Zeng,2 Aiguo Wu,2 and Eric Brown1,*. Dye Surface Coating Enables Visible Light Activation of TiO2 Nanoparticles Leading to Degradation of Neighboring Biological Structures[J]. Microscopy and Microanalysis,2012(18):134—142.
APA	Eric Brown,&Jay Blatnik,1,† Lanette Luebke,1,† Stephanie Simonet,1 Megan Nelson,1 Race Price,1 Rachael Leek,1 Leyong Zeng,2 Aiguo Wu,2 and Eric Brown1,*.(2012).Dye Surface Coating Enables Visible Light Activation of TiO2 Nanoparticles Leading to Degradation of Neighboring Biological Structures.Microscopy and Microanalysis(18),134—142.
MLA	Eric Brown,et al."Dye Surface Coating Enables Visible Light Activation of TiO2 Nanoparticles Leading to Degradation of Neighboring Biological Structures".Microscopy and Microanalysis .18(2012):134—142.