Genetic analysis with nanoPCR

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	Genetic analysis with nanoPCR
	Pan, D(潘敦) ; Mi, LJ(米丽娟) ; Huang, Q(黄庆) ; Hu, J(胡钧) ; Fan, CH(樊春海)
刊名	INTEGRATIVE BIOLOGY
	2012
卷号	4 期号:10
ISSN号	1757-9694
英文摘要	Polymerase chain reaction (PCR) has become a standard and important molecular biological technique with numerous applications in genetic analysis, forensics and in vitro diagnostics. Since its invention in the 1980s, there has been dramatic performance improvement arising from long-lasting efforts to optimize amplification conditions in both academic studies and commercial applications. More recently, a range of nanometer-sized materials including metal nanoparticles, semiconductor quantum dots, carbon nanomaterials and polymer nanoparticles, have shown unique effects in tuning amplification processes of PCR. It is proposed that these artificial nanomaterials mimic protein components in the natural DNA replication machinery in vivo. These so-called nanomaterials-assisted PCR (nanoPCR) strategies shed new light on powerful PCR with unprecedented sensitivity, selectivity and extension rate. In this review, we aim to summarize recent progress in this direction and discuss possible mechanisms for such performance improvement and potential applications in genetic analysis (particularly gene typing and haplotyping) and diagnostics.
语种	英语
WOS记录号	WOS:000309127600001
公开日期	2013-12-05
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/13174]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Pan, D,Mi, LJ,Huang, Q,et al. Genetic analysis with nanoPCR[J]. INTEGRATIVE BIOLOGY,2012,4(10).
APA	Pan, D,Mi, LJ,Huang, Q,Hu, J,&Fan, CH.(2012).Genetic analysis with nanoPCR.INTEGRATIVE BIOLOGY,4(10).
MLA	Pan, D,et al."Genetic analysis with nanoPCR".INTEGRATIVE BIOLOGY 4.10(2012).