Determination of inorganic anions in saliva by electroosmotic flow controlled counterflow isotachophoretic stacking under field-amplified sample injection

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Determination of inorganic anions in saliva by electroosmotic flow controlled counterflow isotachophoretic stacking under field-amplified sample injection
	Ren HX(任海霞)1,2,3 ; Qiu HD(邱洪灯)1,2 ; Liang XJ(梁晓静)1,2 ; Wang XS(王旭生)1,2 ; Jiang SX(蒋生祥)1,2 ; Jiang SX(蒋生祥)
刊名	Journal of Chromatography B
	2013
卷号	935 页码:75-79
关键词	Capillary ion electrophoresis Electrokinetic injection Preconcentration Stacking
ISSN号	1570-0232
通讯作者	蒋生祥
英文摘要	Under a strong counter-electroosmotic flow, five salivary inorganic anions, bromide, iodide, nitrite, nitrate and thiocyanate were determined by field-amplified sample injection in combination with isotachophoretic stacking. Separation and concentration conditions were investigated. A terminating electrolyte, 5 mM borate, was added in the sample. Under the optimized conditions, Br⁻, I⁻and SCN⁻were concentrated online using 150 mM HCl–Tris buffer at pH 7.8 in a bare fused capillary, providing morethan ten thousand of sensitivity enrichment compared with normal injections. The relative standard deviations (RSDs, n = 5) were less than 1% in migration times, 8% in peak areas. Using direct UV detection at 200 nm and 226 nm, the limits of detection (LODs, S/N = 3) were of 0.002–0.01 μM. Unfortunately, NO₂⁻and NO₃⁻could be observed in purified or deionized water. Therefore, a low dilution factor was applied to saliva samples. Due to the matrix effect, samples were injected in a shorter time, and standard addition method was applied to determine all the five inorganic anions in saliva. The RSDs of the migration times and peak areas were in a range of 0.2–0.4% and 3.0–4.0%, respectively. The LODs were 0.2–2.0 μM. The salivary levels of the anions obtained were in accord with the reference data. The external standard method can not be adapted to real samples due to biases caused by electrokinetic injection and errors from high dilutions.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the Natural Science Foundation,China (21105107;21175143);the National Science & Technol-ogy Major Project, China (2011ZX05010;2011ZX05011-004)
语种	英语
WOS记录号	WOS:000324084200012
公开日期	2013-12-10
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/4618]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Jiang SX(蒋生祥)
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ren HX,Qiu HD,Liang XJ,et al. Determination of inorganic anions in saliva by electroosmotic flow controlled counterflow isotachophoretic stacking under field-amplified sample injection[J]. Journal of Chromatography B,2013,935:75-79.
APA	Ren HX,Qiu HD,Liang XJ,Wang XS,Jiang SX,&蒋生祥.(2013).Determination of inorganic anions in saliva by electroosmotic flow controlled counterflow isotachophoretic stacking under field-amplified sample injection.Journal of Chromatography B,935,75-79.
MLA	Ren HX,et al."Determination of inorganic anions in saliva by electroosmotic flow controlled counterflow isotachophoretic stacking under field-amplified sample injection".Journal of Chromatography B 935(2013):75-79.