Simultaneous determination of Six alkaloids in phellodendron amurense by high-performance liquid chromatography | |
Li CD(李彩东)1,2,3; Liu Y(刘勇)1,2; Shi YP(师彦平)1,2![]() | |
刊名 | Acta Chromatographia
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2013 | |
卷号 | 25期号:2页码:275-285 |
关键词 | high-performance liquid chromatography phytochemical analysis traditional Chinese medicine Phellodendron amurense alkaloids |
ISSN号 | 1233-2356 |
通讯作者 | 师彦平 |
英文摘要 | A high-performance liquid chromatography (HPLC) method has been developed for simultaneous determination of six alkaloids, i.e., (−)-(R)-platydesmin, noroxyhydrastinine, berberine, skimmianine, canthin-6-one, and pteleine in the herbal medicine of Phellodendron amurense Rupr. The optimal condition for extraction and separation was achieved with a linear mobile phase gradient prepared from 0.1% phosphoric acid and acetonitrile. The LODs and LOQs for the analytes ranged from 0.06 to 0.22 μg mL−1 and from 0.25 to 0.80 μg mL−1, respectively. The optimized method was applied to the determination of alkaloids in P. amurense Rupr. and was found to be efficient. This method can provide a scientific and technical platform to the manufacturers for setting up a quality control standard as well as to the public for quality and safety assurance of the proprietary traditional Chinese medicines. |
学科主题 | 分析化学与药物化学 |
收录类别 | SCI |
资助信息 | the National Key Technology Research and Development Program of China (no. 2007BAI37B05);the National Natural Science Foundation of China (nos. 20875095;21075127) |
语种 | 英语 |
WOS记录号 | WOS:000319748700005 |
公开日期 | 2013-12-10 |
内容类型 | 期刊论文 |
源URL | [http://210.77.64.217/handle/362003/4583] ![]() |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Li CD,Liu Y,Shi YP. Simultaneous determination of Six alkaloids in phellodendron amurense by high-performance liquid chromatography[J]. Acta Chromatographia,2013,25(2):275-285. |
APA | Li CD,Liu Y,&Shi YP.(2013).Simultaneous determination of Six alkaloids in phellodendron amurense by high-performance liquid chromatography.Acta Chromatographia,25(2),275-285. |
MLA | Li CD,et al."Simultaneous determination of Six alkaloids in phellodendron amurense by high-performance liquid chromatography".Acta Chromatographia 25.2(2013):275-285. |
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