Characterization and modulation of the unimolecular conformation of integrins with nanopore sensors | |
Li, Rongjie6; Liu, Daixin4,5; Ren, Meili3,4,5; Qin, Fupeng4,5; Qi, Guodong2; Tian, Dawei6; Zhang, Mingkun1,4,5; Xie, Wanyi4,5; Wang, Liang4,5; Lu, Shouqin1 | |
刊名 | CHEMICAL ENGINEERING JOURNAL |
2024-07-15 | |
卷号 | 492页码:8 |
关键词 | Single molecule integrin Solid-state nanopores divalent metal ions Oligopeptides Conformation |
ISSN号 | 1385-8947 |
DOI | 10.1016/j.cej.2024.152374 |
通讯作者 | Pan, Jun(panj@cqu.edu.cn) ; Liang, Liyuan(liangliyuan@cigit.ac.cn) |
英文摘要 | Integrins are a large family of heterodimeric mammalian transmembrane receptors that connect extracellular matrix (ECM) and the cytoskeleton. Integrin-mediated adhesion governs the cellular adhesion, mobility, and cell mechanobiology. Investigations on different integrins in cellular and the conformation change regulated by divalent metal ions and oligopeptides have been documented, they mainly adopted the cellular assays or surface manipulation to achieve the information of ensemble integrins with proteins and small molecules. This work presents the nanopore approach for the characterization of unimolecular integrin via resistive pulse sensing. The orientation selection in distinct nanopore in size and pH condition discloses the tiny disparity of the conformation and the local surface charge distribution. The divalent metal ions could efficiently modulate the conformation alteration of integrin, in which Ca2+ and Mn2+ could activate integrin alpha L beta 2 to take larger physical dimension than Mg2+, and Ca2+ is superior to Mn2+ in the activation when integrin alpha L beta 2 is treated in the mixed metal ions. The oligopeptide cRGD (cyclic Arg-Gly-Asp) and its derivatives cRGDfk and cRGDyk show specific binding with integrin alpha V beta 3 and the complex of alpha V beta 3-cRGD exhibits largest geometry upon Ca2+-mediation with characteristic nanopore translocation feature. This work provides a platform for unimolecular integrin sensing and the modulation of the local conformation transition regulated by divalent metal ions and oligopeptides, which will be valuable for the representation of integrin-related cellular behavior and bio-function. |
资助项目 | Excellent Youth Foundation of Chongqing Scientific Committee[cstc2021jcyj-jqX0030] ; West Light Foundation of the Chinese Academy of Sciences ; National Key Research and Development Program of China[2022YFB3205600] ; University of the Chinese Academy of Sciences |
WOS关键词 | ACTIVATION |
WOS研究方向 | Engineering |
语种 | 英语 |
WOS记录号 | WOS:001244390500001 |
资助机构 | Excellent Youth Foundation of Chongqing Scientific Committee ; West Light Foundation of the Chinese Academy of Sciences ; National Key Research and Development Program of China ; University of the Chinese Academy of Sciences |
内容类型 | 期刊论文 |
源URL | [http://dspace.imech.ac.cn/handle/311007/95672] |
专题 | 力学研究所_国家微重力实验室 |
通讯作者 | Pan, Jun; Liang, Liyuan |
作者单位 | 1.Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China 2.Northeast Elect Power Univ, Sch Chem Engn, Jilin 132012, Jilin, Peoples R China 3.Chongqing Jiaotong Univ, Chongqing 400014, Peoples R China 4.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China 5.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 6.Chongqing Univ, Coll Bioengn 10, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants,Minist, Chongqing 400044, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Rongjie,Liu, Daixin,Ren, Meili,et al. Characterization and modulation of the unimolecular conformation of integrins with nanopore sensors[J]. CHEMICAL ENGINEERING JOURNAL,2024,492:8. |
APA | Li, Rongjie.,Liu, Daixin.,Ren, Meili.,Qin, Fupeng.,Qi, Guodong.,...&Liang, Liyuan.(2024).Characterization and modulation of the unimolecular conformation of integrins with nanopore sensors.CHEMICAL ENGINEERING JOURNAL,492,8. |
MLA | Li, Rongjie,et al."Characterization and modulation of the unimolecular conformation of integrins with nanopore sensors".CHEMICAL ENGINEERING JOURNAL 492(2024):8. |
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