Advances in environmental DNA monitoring: standardization, automation, and emerging technologies in aquatic ecosystems

doi:10.1007/s11427-023-2493-5

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海鱼类物种与基因组多样性的研究

	Advances in environmental DNA monitoring: standardization, automation, and emerging technologies in aquatic ecosystems
	Lu, Suxiang 5; Zeng, Honghui 5; Xiong, Fan 5; Yao, Meng 3,4; He, Shunping 1,2,5
刊名	SCIENCE CHINA-LIFE SCIENCES
	2024-03-18
页码	17
关键词	eDNA methodology sampler environment biomonitoring ecogenomic sensors ocean observation instrument
ISSN号	1674-7305
DOI	10.1007/s11427-023-2493-5
英文摘要	Environmental DNA (eDNA) monitoring, a rapidly advancing technique for assessing biodiversity and ecosystem health, offers a noninvasive approach for detecting and quantifying species from various environmental samples. In this review, a comprehensive overview of current eDNA collection and detection technologies is provided, emphasizing the necessity for standardization and automation in aquatic ecological monitoring. Furthermore, the intricacies of water bodies, from streams to the deep sea, and the associated challenges they pose for eDNA capture and analysis are explored. The paper delineates three primary eDNA survey methods, namely, bringing back water, bringing back filters, and bringing back data, each with specific advantages and constraints in terms of labor, transport, and data acquisition. Additionally, innovations in eDNA sampling equipment, including autonomous drones, subsurface samplers, and in-situ filtration devices, and their applications in monitoring diverse taxa are discussed. Moreover, recent advancements in species-specific detection and eDNA metabarcoding are addressed, highlighting the integration of novel techniques such as CRISPR-Cas and nanopore sequencing that enable precise and rapid detection of biodiversity. The implications of environmental RNA and epigenetic modifications are considered for future applications in providing nuanced ecological data. Lastly, the review stresses the critical role of standardization and automation in enhancing data consistency and comparability for robust long-term biomonitoring. We propose that the amalgamation of these technologies represents a paradigm shift in ecological monitoring, aligning with the urgent call for biodiversity conservation and sustainable management of aquatic ecosystems.
资助项目	National Natural Science Foundation of China[32325034] ; National Natural Science Foundation of China[U2340216] ; National Key Research and Development Program of China[2022YFF0608200] ; Special Project for Social Development of Yunnan Province[202103AC100001] ; Wuhan Branch, Supercomputing Center of CAS
WOS关键词	EDNA DETECTION ; HARMFUL ALGAE ; PCR ; TOOL ; AMPLIFICATION ; CONSERVATION ; INHIBITION ; PATTERNS ; MARIANA ; MARKERS
WOS研究方向	Life Sciences & Biomedicine - Other Topics
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:001190075200001
资助机构	National Natural Science Foundation of China ; National Key Research and Development Program of China ; Special Project for Social Development of Yunnan Province ; Wuhan Branch, Supercomputing Center of CAS
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10953]
专题	深海科学研究部_深海生物学研究室_深海鱼类物种与基因组多样性的研究
通讯作者	Yao, Meng; He, Shunping
作者单位	1.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming, Peoples R China 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Peoples R China 3.Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China 4.Peking Univ, Inst Ecol, Coll Urban & Environm Sci, Beijing 100871, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Lu, Suxiang,Zeng, Honghui,Xiong, Fan,et al. Advances in environmental DNA monitoring: standardization, automation, and emerging technologies in aquatic ecosystems[J]. SCIENCE CHINA-LIFE SCIENCES,2024:17.
APA	Lu, Suxiang,Zeng, Honghui,Xiong, Fan,Yao, Meng,&He, Shunping.(2024).Advances in environmental DNA monitoring: standardization, automation, and emerging technologies in aquatic ecosystems.SCIENCE CHINA-LIFE SCIENCES,17.
MLA	Lu, Suxiang,et al."Advances in environmental DNA monitoring: standardization, automation, and emerging technologies in aquatic ecosystems".SCIENCE CHINA-LIFE SCIENCES (2024):17.