Scallop-bacteria symbiosis from the deep sea reveals strong genomic coupling in the absence of cellular integration

doi:10.1093/ismejo/wrae048

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋微生物宏基因组与分子进化研究组

	Scallop-bacteria symbiosis from the deep sea reveals strong genomic coupling in the absence of cellular integration
	Lin, Yi-Tao 7; Ip, Jack Chi-Ho 6; He, Xing 5; Gao, Zhao-Ming 4; Perez, Maeva 7; Xu, Ting 2,3; Sun, Jin 5; Qian, Pei-Yuan 2,3; Qiu, Jian-Wen 1,7
刊名	ISME JOURNAL
	2024-01-08
卷号	18 期号:1 页码:14
关键词	chemosynthesis cold seep ectosymbiosis glass scallop thiotrophy
ISSN号	1751-7362
DOI	10.1093/ismejo/wrae048
英文摘要	Previous studies have revealed tight metabolic complementarity between bivalves and their endosymbiotic chemosynthetic bacteria, but little is known about their interactions with ectosymbionts. Our analysis of the ectosymbiosis between a deep-sea scallop (Catillopecten margaritatus) and a gammaproteobacterium showed that bivalves could be highly interdependent with their ectosymbionts as well. Our microscopic observation revealed abundant sulfur-oxidizing bacteria (SOB) on the surfaces of the gill epithelial cells. Microbial 16S rRNA gene amplicon sequencing of the gill tissues showed the dominance of the SOB. An analysis of the SOB genome showed that it is substantially smaller than its free-living relatives and has lost cellular components required for free-living. Genomic and transcriptomic analyses showed that this ectosymbiont relies on rhodanese-like proteins and SOX multienzyme complex for energy generation, mainly on the Calvin-Benson-Bassham (CBB) cycle and peripherally on a phosphoenolpyruvate carboxylase for carbon assimilation. Besides, the symbiont encodes an incomplete tricarboxylic acid (TCA) cycle. Observation of the scallop's digestive gland and its nitrogen metabolism pathways indicates it does not fully rely on the ectosymbiont for nutrition. Analysis of the host's gene expression provided evidence that it could offer intermediates for the ectosymbiont to complete its TCA cycle and some amino acid synthesis pathways using exosomes, and its phagosomes, endosomes, and lysosomes might be involved in harvesting nutrients from the symbionts. Overall, our study prompts us to rethink the intimacy between the hosts and ectosymbionts in Bivalvia and the evolution of chemosymbiosis in general.
资助项目	Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[SMSEGL24SC01] ; Fundamental Research Funds for the Central Universities[202172002] ; Fundamental Research Funds for the Central Universities[202241002] ; National Key R&D Program of China[2022YFC2805505] ; Hong Kong SAR[C2013-22GF] ; Hong Kong SAR[16101822] ; Hong Kong SAR[12101021] ; Hong Kong SAR[12102623]
WOS关键词	READ ALIGNMENT ; METHANE-SEEP ; SEQUENCE ; MUSSEL ; TRANSMISSION ; EVOLUTION ; BIVALVIA ; RECONSTRUCTION ; PECTINIDAE ; GENERATION
WOS研究方向	Environmental Sciences & Ecology ; Microbiology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:001197874200001
资助机构	Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) ; Fundamental Research Funds for the Central Universities ; National Key R&D Program of China ; Hong Kong SAR
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10974]
专题	深海科学研究部_深海生物学研究室_海洋微生物宏基因组与分子进化研究组
通讯作者	Qiu, Jian-Wen
作者单位	1.Hong Kong Baptist Univ, Dept Biol, Kowloon Tong, RRS820,224 Waterloo Rd, Hong Kong, Peoples R China 2.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Hong Kong 999077, Peoples R China 3.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou 511458, Peoples R China 4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Deep Sea Sci Div, Sanya 572000, Peoples R China 5.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China 6.Lingnan Univ, Sci Unit, Hong Kong 999077, Peoples R China 7.Hong Kong Baptist Univ, Dept Biol, Hong Kong 999077, Peoples R China
推荐引用方式 GB/T 7714	Lin, Yi-Tao,Ip, Jack Chi-Ho,He, Xing,et al. Scallop-bacteria symbiosis from the deep sea reveals strong genomic coupling in the absence of cellular integration[J]. ISME JOURNAL,2024,18(1):14.
APA	Lin, Yi-Tao.,Ip, Jack Chi-Ho.,He, Xing.,Gao, Zhao-Ming.,Perez, Maeva.,...&Qiu, Jian-Wen.(2024).Scallop-bacteria symbiosis from the deep sea reveals strong genomic coupling in the absence of cellular integration.ISME JOURNAL,18(1),14.
MLA	Lin, Yi-Tao,et al."Scallop-bacteria symbiosis from the deep sea reveals strong genomic coupling in the absence of cellular integration".ISME JOURNAL 18.1(2024):14.